Chemical synthesis of erythropoietin glycoforms for insights into the relationship between glycosylation pattern and bioactivity - Murakami, Masumi; Kiuchi, Tatsuto; Nishihara, Mika; Tezuka, Katsunari; Okamoto, Ryo; Izumi, Masayuki; Kajihara, Yasuhiro (Science Advances, 2016)

Reconciling past changes in Earth’s rotation with 20th century global sea-level rise: Resolving Munk’s enigma - Mitrovica, Jerry X.; Hay, Carling C.; Morrow, Eric; Kopp, Robert E.; Dumberry, Mathieu; Stanley, Sabine (Science Advances, 2015)

Nutritional input from dinoflagellate symbionts in reef-building corals is minimal during planula larval life stage - Kopp, Christophe; Domart-Coulon, Isabelle; Barthelemy, Dominique; Meibom, Anders (Science Advances, 2016)

Past and future rainfall in the Horn of Africa - Tierney, Jessica E.; Ummenhofer, Caroline C.; deMenocal, Peter B. (Science Advances, 2015)

Native chemical ligation at a base-labile 4-mercaptobutyrate Nα-auxiliary - Harpaz, Ziv; Loibl, Simon; Seitz, Oliver (Bioorganic & Medicinal Chemistry Letters, 2016)
Keywords: #nosource

Determinants of High-Affinity Binding and Receptor Activation in the N-Terminus of CCL-19 (MIP-3β) - Ott, T. R.; Lio, F. M.; Olshefski, D.; Liu, X.-J.; Struthers, R. S.; Ling, N. (Biochemistry, 2004)
Keywords: #nosource

Protein chemical synthesis by α-ketoacid–hydroxylamine ligation - Harmand, Thibault J; Murar, Claudia E; Bode, Jeffrey W (Nature Protocols, 2016)
Keywords: #nosource

Chemical Synthesis of the 20 kDa Heme Protein Nitrophorin 4 by α-Ketoacid-Hydroxylamine (KAHA) Ligation - He, Chunmao; Kulkarni, Sameer S.; Thuaud, Frédéric; Bode, Jeffrey W. (Angewandte Chemie International Edition, 2015)
Keywords: #nosource

Chemical synthesis of a homoserine-mutant of the antibacterial, head-to-tail cyclized protein AS-48 by α-ketoacid–hydroxylamine (KAHA) ligation - Rohrbacher, Florian; Zwicky, André; Bode, Jeffrey W. (Chemical Science, 2017)

Chemical Protein Synthesis by Chemoselective α-Ketoacid-Hydroxylamine (KAHA) Ligations with 5-Oxaproline - Pattabiraman, Vijaya R.; Ogunkoya, Ayodele O.; Bode, Jeffrey W. (Angewandte Chemie, 2012)
Keywords: #nosource

Synthesis of human GLP-1 (7–36) by chemoselective α-ketoacid–hydroxylamine peptide ligation of unprotected fragments - Wu, Jian; Ruiz-Rodríguez, Javier; Comstock, Jeanne M.; Dong, Jesse Z.; Bode, Jeffrey W. (Chemical Science, 2011)
Keywords: #nosource

Chemical Synthesis of the 12 kDa Human Myokine Irisin by α -Ketoacid-Hydroxylamine (KAHA) Ligation - Wucherpfennig, Thomas G.; Müller, Sebastian; Wolfrum, Christian; Bode, Jeffrey W. (Helvetica Chimica Acta, 2016)
Keywords: #nosource

Photoprotected Peptide α -Ketoacids and Hydroxylamines for Iterative and One-Pot KAHA Ligations: Synthesis of NEDD8 - Thuaud, Frédéric; Rohrbacher, Florian; Zwicky, André; Bode, Jeffrey W. (Helvetica Chimica Acta, 2016)
Keywords: #nosource

Chemical Synthesis of HMGA1a Proteins with Post-translational Modifications via Ser/Thr Ligation - Li, Tianlu; Liu, Heng; Li, Xuechen (Organic Letters, 2016)
Keywords: #nosource

In vitro and in cell analysis of chemically synthesized histone H2A with multiple modifications - Hayashi, Gosuke; Sueoka, Takuma; Okamoto, Akimitsu (Chemical Communications, 2016)
Keywords: #nosource

Semisynthesis of Peptoid–Protein Hybrids by Chemical Ligation at Serine - Levine, Paul M.; Craven, Timothy W.; Bonneau, Richard; Kirshenbaum, Kent (Organic Letters, 2014)
Keywords: #nosource

Synthesis of human growth hormone-releasing hormone via three-fragment serine/threonine ligation (STL) - Zhang, Yinfeng; Li, Tianlu; Li, Xuechen (Organic & Biomolecular Chemistry, 2013)
Keywords: #nosource

Facile Synthesis of Peptidyl Salicylaldehyde Esters and Its Use in Cyclic Peptide Synthesis - Zhao, Jun-Feng; Zhang, Xiao-Hong; Ding, Ying-Jie; Yang, Yong-Sheng; Bi, Xiao-Bao; Liu, Chuan-Fa (Organic Letters, 2013)
Keywords: #nosource

Effective synthesis of kynurenine-containing peptides via on-resin ozonolysis of tryptophan residues: synthesis of cyclomontanin B - Wong, Clarence T. T.; Lam, Hiu Yung; Li, Xuechen (Organic & Biomolecular Chemistry, 2013)
Keywords: #nosource

Effective synthesis of cyclic peptide yunnanin C and analogues via Ser/Thr ligation (STL)-mediated peptide cyclization - Wong, Clarence T.T.; Lam, Hiu Yung; Li, Xuechen (Tetrahedron, 2014)
Keywords: #nosource

Chemical synthesis and enzymatic properties of RNase A analogues designed to enhance second-step catalytic activity - Boerema, David J.; Tereshko, Valentina A.; Zhang, Junliang; Kent, Stephen B. H. (Organic & Biomolecular Chemistry, 2016)
Keywords: #nosource

Development of a strategy for the total chemical synthesis of an allergenic protein: the peach LTP Pru p 3: CHEMICAL SYNTHESIS OF THE ALLERGENIC PEACH LIPID TRANSFER PROTEIN - Buhler, Sofie; Akkerdaas, Jaap H.; A. Pertinhez, Thelma; Van Ree, Ronald; Dossena, Arnaldo; Sforza, Stefano; Tedeschi, Tullia (Journal of Peptide Science, 2017)
Keywords: #nosource

Elucidation of the Covalent and Tertiary Structures of Biologically Active Ts3 Toxin - Dang, Bobo; Kubota, Tomoya; Mandal, Kalyaneswar; Correa, Ana M.; Bezanilla, Francisco; Kent, Stephen B. H. (Angewandte Chemie International Edition, 2016)

Chemical Synthesis and Characterization of an Equinatoxin II(1–85) Analogue - Karas, John; Sani, Marc-Antoine; Separovic, Frances (Molecules, 2017)

5-Methylisoxazole-3-carboxamide-Directed Palladium-Catalyzed γ-C(sp 3 )–H Acetoxylation and Application to the Synthesis of γ-Mercapto Amino Acids for Native Chemical Ligation - Pasunooti, Kalyan Kumar; Yang, Renliang; Banerjee, Biplab; Yap, Terence; Liu, Chuan-Fa (Organic Letters, 2016)
Keywords: #nosource

Total chemical synthesis of a biologically active and homogeneous analog of Human Growth Hormone [Nle 14,125,170 ,Glu 29,91 ,Gln 74 ,Asn 107 ,Asp 109 ]hGH-NH 2 by sequential native chemical ligation - Sueiras-Diaz, Javier; Zhang, Ying; Velentza, Anastasia; Santoso, Buyung; Yang, Suijin (Tetrahedron Letters, 2017)
Keywords: #nosource

Synthesis of Grb2 SH2 Domain Proteins for Mirror-Image Screening Systems - Noguchi, Taro; Ishiba, Hiroyuki; Honda, Kaori; Kondoh, Yasumitsu; Osada, Hiroyuki; Ohno, Hiroaki; Fujii, Nobutaka; Oishi, Shinya (Bioconjugate Chemistry, 2017)

Total Chemical Synthesis and Folding of All- l and All- d Variants of Oncogenic KRas(G12V) - Levinson, Adam M.; McGee, John H.; Roberts, Andrew G.; Creech, Gardner S.; Wang, Ting; Peterson, Michael T.; Hendrickson, Ronald C.; Verdine, Gregory L.; Danishefsky, Samuel J. (Journal of the American Chemical Society, 2017)

Synthesis of misfolded glycoprotein dimers through native chemical ligation of a dimeric peptide thioester - Izumi, Masayuki; Komaki, Shinji; Okamoto, Ryo; Seko, Akira; Takeda, Yoichi; Ito, Yukishige; Kajihara, Yasuhiro (Organic & Biomolecular Chemistry, 2016)
Keywords: #nosource

Chemical Synthesis of Glycoproteins with the Specific Installation of Gradient-Enriched 15 N-Labeled Amino Acids for Getting Insights into Glycoprotein Behavior - Minh Hien, Nguyen; Izumi, Masayuki; Sato, Hajime; Okamoto, Ryo; Kajihara, Yasuhiro (Chemistry - A European Journal, 2017)
Keywords: #nosource

Convergent Glycopeptide Synthesis by Traceless Staudinger Ligation and Enzymatic Coupling - Liu, Lei; Hong, Zhang-Yong; Wong, Chi-Huey (ChemBioChem, 2006)
Keywords: #nosource

Semi‐synthesis of murine prion protein by native chemical ligation and chemical activation for preparation of polypeptide‐ α ‐thioester - Shi, Lei; Chen, Huai; Zhang, Si‐Yu; Chu, Ting‐Ting; Zhao, Yu‐Fen; Chen, Yong‐Xiang; Li, Yan‐Mei (Journal of Peptide Science, 2017)
Keywords: #nosource

The convergent chemical synthesis of histone H3 protein for site-specific acetylation at Lys56 and ubiquitination at Lys122 - Qi, Yun-Kun; He, Qiao-Qiao; Ai, Hua-Song; Guo, Jing; Li, Jia-Bin (Chemical Communications, 2017)
Keywords: #nosource

Total chemical synthesis of methylated analogues of histone 3 revealed KDM4D as a potential regulator of H3K79me3 - Jbara, Muhammad; Guttmann-Raviv, Noga; Maity, Suman Kumar; Ayoub, Nabieh; Brik, Ashraf (Bioorganic & Medicinal Chemistry, 2017)
Keywords: #nosource

Total chemical synthesis of SUMO-2-Lys63-linked diubiquitin hybrid chains assisted by removable solubilizing tags - Bondalapati, Somasekhar; Eid, Emad; Mali, Sachitanand M.; Wolberger, Cynthia; Brik, Ashraf (Chemical Science, 2017)

Chemical synthesis of histone H2A with methylation at Gln104 - He, Qiaoqiao; Li, Jiabin; Qi, Yunkun; Wang, Zhipeng; Huang, Yong; Liu, Lei (Science China Chemistry, 2017)
Keywords: #nosource

Immobilization methods for the rapid total chemical synthesis of proteins on microtiter plates: Rapid Chemical Synthesis of Proteins on Microtiter Plates - Zitterbart, Robert; Krumrey, Michael; Seitz, Oliver (Journal of Peptide Science, 2017)
Keywords: #nosource

Rapid Additive-Free Selenocystine–Selenoester Peptide Ligation - Mitchell, Nicholas J.; Malins, Lara R.; Liu, Xuyu; Thompson, Robert E.; Chan, Bun; Radom, Leo; Payne, Richard J. (Journal of the American Chemical Society, 2015)
Keywords: #nosource

A Helping Hand to Overcome Solubility Challenges in Chemical Protein Synthesis - Jacobsen, Michael T.; Petersen, Mark E.; Ye, Xiang; Galibert, Mathieu; Lorimer, George H.; Aucagne, Vincent; Kay, Michael S. (Journal of the American Chemical Society, 2016)

Investigation of Cysteine as an Activator of Side-Chain N→S Acyl Transfer and Tail-to-Side-Chain Cyclization - Castillo-Pazos, Durbis; Macmillan, Derek (Synlett, 2017)
Keywords: #nosource

Semisynthetic prion protein (PrP) variants carrying glycan mimics at position 181 and 197 do not form fibrils - Araman, Can; Thompson, Robert E.; Wang, Siyao; Hackl, Stefanie; Payne, Richard J.; Becker, Christian F. W. (Chemical Science, 2017)

Chemical synthesis of dual labeled proteins via differently protected alkynes enables intramolecular FRET analysis - Hayashi, Gosuke; Kamo, Naoki; Okamoto, Akimitsu (Chemical Communications, 2017)
Keywords: #nosource

A semisynthetic Atg3 reveals that acetylation promotes Atg3 membrane binding and Atg8 lipidation - Li, Yi-Tong; Yi, Cong; Chen, Chen-Chen; Lan, Huan; Pan, Man; Zhang, Shao-Jin; Huang, Yi-Chao; Guan, Chao-Jian; Li, Yi-Ming; Yu, Li; Liu, Lei (Nature Communications, 2017)

Chemical Synthesis of Diubiquitin-Based Photoaffinity Probes for Selectively Profiling Ubiquitin-Binding Proteins - Liang, Jun; Zhang, Lin; Tan, Xiang-Long; Qi, Yun-Kun; Feng, Shan; Deng, Haiteng; Yan, Yijing; Zheng, Ji-Shen; Liu, Lei; Tian, Chang-Lin (Angewandte Chemie International Edition, 2017)
Keywords: #nosource

Chemical Synthesis of K34-Ubiquitylated H2B for Nucleosome Reconstitution and Single-Particle Cryo-Electron Microscopy Structural Analysis - Li, Jiabin; He, Qiaoqiao; Liu, Yuntao; Liu, Sanling; Tang, Shan; Li, Chengmin; Sun, Demeng; Li, Xiaorun; Zhou, Min; Zhu, Ping; Bi, Guoqiang; Zhou, Zhenghong; et al. (ChemBioChem, 2017)
Keywords: #nosource

Metal-Assisted Folding of Prolinomycin Allows Facile Design of Functional Peptides - Hosseini, Azade S.; Wang, Wenjian; Haeffner, Fredrik; Gao, Jianmin (ChemBioChem, 2017)

Synthesis of the Src SH2 domain and its application in bioassays for mirror-image screening - Shu, Keitou; Noguchi, Taro; Honda, Kaori; Kondoh, Yasumitsu; Osada, Hiroyuki; Ohno, Hiroaki; Fujii, Nobutaka; Oishi, Shinya (RSC Advances, 2017)

Acceleration of thiol additive-free native chemical ligation by intramolecular S → S acyl transfer - Schmalisch, J.; Seitz, O. (Chemical Communications, 2015)
Keywords: #nosource

Europium-Labeled Synthetic C3a Protein as a Novel Fluorescent Probe for Human Complement C3a Receptor - Dantas de Araujo, Aline; Wu, Chongyang; Wu, Kai-Chen; Reid, Robert C.; Durek, Thomas; Lim, Junxian; Fairlie, David P. (Bioconjugate Chemistry, 2017)

Scope and Limitations of Fmoc Chemistry SPPS-Based Approaches to the Total Synthesis of Insulin Lispro via Ester Insulin - Dhayalan, Balamurugan; Mandal, Kalyaneswar; Rege, Nischay; Weiss, Michael A.; Eitel, Simon H.; Meier, Thomas; Schoenleber, Ralph O.; Kent, Stephen B. H. (Chemistry - A European Journal, 2017)

Full Sequence Amino Acid Scanning of θ-Defensin RTD-1 Yields a Potent Anthrax Lethal Factor Protease Inhibitor - Li, Yilong; Gould, Andrew; Aboye, Teshome; Bi, Tao; Breindel, Leonard; Shekhtman, Alexander; Camarero, Julio A. (Journal of Medicinal Chemistry, 2017)

Discovery of a Novel Series of Orally Bioavailable and CNS Penetrant Glucagon-like Peptide-1 Receptor (GLP-1R) Noncompetitive Antagonists Based on a 1,3-Disubstituted-7-aryl-5,5-bis(trifluoromethyl)-5,8-dihydropyrimido[4,5- d ]pyrimidine-2,4(1 H ,3 H )-dione Core - Nance, Kellie D.; Days, Emily L.; Weaver, C. David; Coldren, Anastasia; Farmer, Tiffany D.; Cho, Hyekyung P.; Niswender, Colleen M.; Blobaum, Anna L.; Niswender, Kevin D.; Lindsley, Craig W. (Journal of Medicinal Chemistry, 2017)
Keywords: #nosource

P−B Desulfurization: An Enabling Method for Protein Chemical Synthesis and Site-Specific Deuteration - Jin, Kang; Li, Tianlu; Chow, Hoi Yee; Liu, Han; Li, Xuechen (Angewandte Chemie International Edition, 2017)
Keywords: #nosource

Synthesis of a secretoglobin 3A2 type C (98–139) fragment by Dawson’s native chemical ligation - Kikuchi, Mariko; Kurotani, Reiko; Konno, Hiroyuki (Tetrahedron Letters, 2017)
Keywords: #nosource

A novel ring opening reaction of peptide N-terminal thiazolidine with 2,2′-dipyridyl disulfide (DPDS) efficient for protein chemical synthesis - Katayama, Hidekazu; Morisue, Satoki (Tetrahedron, 2017)
Keywords: #nosource

O-GlcNAc modification inhibits the calpain-mediated cleavage of α-synuclein - Levine, Paul M.; De Leon, Cesar A.; Galesic, Ana; Balana, Aaron; Marotta, Nicholas P.; Lewis, Yuka E.; Pratt, Matthew R. (Bioorganic & Medicinal Chemistry, 2017)

Divergent Protein Synthesis of Bowman–Birk Protease Inhibitors, their Hydrodynamic Behavior and Co-crystallization with α-Chymotrypsin - Tornøe, Christian; Johansson, Eva; Wahlund, Per-Olof (Synlett, 2017)
Keywords: #nosource

Ag(I)-Catalyzed Cycloisomerization and Cyclization of Ketene Aminals: Construction of Azepine and 1,2-Dihydropyridine Derivatives - Nayak, Sanatan; Prabagar, B.; Ghosh, Nayan; Mallick, Rajendra; Sahoo, Akhila (Synthesis, 2017)
Keywords: #nosource

Nickel-Catalyzed Hydroalkenylation of Alkynes through C–F Bond Activation: Synthesis of 2-Fluoro-1,3-dienes - Watabe, Yota; Kanazawa, Kohei; Fujita, Takeshi; Ichikawa, Junji (Synthesis, 2017)
Keywords: #nosource

Solid Organozinc Pivalates: A New Class of Zinc Organometallics with Greatly Enhanced Air- and Moisture-Stability - Chen, Yi-Hung; Ellwart, Mario; Malakhov, Vladimir; Knochel, Paul (Synthesis, 2017)
Keywords: #nosource

Synthesis of (E)-4,5-Dihydro-1H-Pyrazoles via Tandem Intermolecular Addition–Cyclization of N-Propargylic Sulfonylhydrazones - Tang, Hai-Tao; Zeng, Jia-Hao; Chen, Jun-Jia; Chen, Yi-Hui; Zhan, Zhuang-Ping (Synlett, 2017)
Keywords: #nosource

Tyrosine sulfation modulates activity of tick-derived thrombin inhibitors - Thompson, Robert E.; Liu, Xuyu; Ripoll-Rozada, Jorge; Alonso-García, Noelia; Parker, Benjamin L.; Pereira, Pedro José Barbosa; Payne, Richard J. (Nature Chemistry, 2017)
Keywords: #nosource

A device that operates within a self-assembled 3D DNA crystal - Hao, Yudong; Kristiansen, Martin; Sha, Ruojie; Birktoft, Jens J.; Hernandez, Carina; Mao, Chengde; Seeman, Nadrian C. (Nature Chemistry, 2017)
Keywords: #nosource

Chlorodifluoromethane-triggered formation of difluoromethylated arenes catalysed by palladium - Feng, Zhang; Min, Qiao-Qiao; Fu, Xia-Ping; An, Lun; Zhang, Xingang (Nature Chemistry, 2017)
Keywords: #nosource

Solvent-switchable continuous-breathing behaviour in a diamondoid metal–organic framework and its influence on CO2 versus CH4 selectivity - Carrington, Elliot J.; McAnally, Craig A.; Fletcher, Ashleigh J.; Thompson, Stephen P.; Warren, Mark; Brammer, Lee (Nature Chemistry, 2017)

Visible-light excitation of iminium ions enables the enantioselective catalytic β-alkylation of enals - Silvi, Mattia; Verrier, Charlie; Rey, Yannick P.; Buzzetti, Luca; Melchiorre, Paolo (Nature Chemistry, 2017)

A polymer nanoparticle with engineered affinity for a vascular endothelial growth factor (VEGF165) - Koide, Hiroyuki; Yoshimatsu, Keiichi; Hoshino, Yu; Lee, Shih-Hui; Okajima, Ai; Ariizumi, Saki; Narita, Yudai; Yonamine, Yusuke; Weisman, Adam C.; Nishimura, Yuri; Oku, Naoto; Miura, Yoshiko; et al. (Nature Chemistry, 2017)
Keywords: #nosource

Total chemical synthesis of ester-linked ubiquitinated proteins unravels their behavior with deubiquitinases - Sun, Hao; Meledin, Roman; Mali, Sachitanand M.; Brik, Ashraf (Chemical Science, 2018)

Regulation of the Stability of the Histone H2A–H2B Dimer by H2A Tyr57 Phosphorylation - Sueoka, Takuma; Hayashi, Gosuke; Okamoto, Akimitsu (Biochemistry, 2017)
Keywords: #nosource

One-pot ligation strategy for atypical ubiquitin chains synthesis by using the trifluoroacetamidomethyl-protected isopeptide-linked Ub (Tfacm-isoUb) unit - Si, Yan-Yan; Liang, Lu-Jun; Tang, Shan; Qi, Yun-Kun; Huang, Yong; Zheng, Ji-Shen (Tetrahedron Letters, 2018)
Keywords: #nosource

Chemical synthesis of membrane proteins by the removable backbone modification method - Tang, Shan; Zuo, Chao; Huang, Dong-Liang; Cai, Xiao-Ying; Zhang, Long-Hua; Tian, Chang-Lin; Zheng, Ji-Shen; Liu, Lei (Nature Protocols, 2017)
Keywords: #nosource

Chemical Synthesis of the Highly Hydrophobic Antiviral Membrane-Associated Protein IFITM3 and Modified Variants - Harmand, Thibault J.; Pattabiraman, Vijaya R.; Bode, Jeffrey W. (Angewandte Chemie International Edition, 2017)

Native chemical ubiquitination using a genetically incorporated azidonorleucine - Yang, Renliang; Bi, Xiaobao; Li, Fupeng; Cao, Yuan; Liu, Chuan-Fa (Chem. Commun., 2014)
Keywords: #nosource

Synthesis of ubiquitylated histone H3 using a thiirane linker for chemical ligation: Synthesis of ubiquitylated histone H3 using a thiirane linker - Kawakami, Toru; Mishima, Yuichi; Hojo, Hironobu; Suetake, Isao (Journal of Peptide Science, 2017)
Keywords: #nosource

Coupling of sterically demanding peptides by β-thiolactone-mediated native chemical ligation - Chen, Huan; Xiao, Yunxian; Yuan, Ning; Weng, Jiaping; Gao, Pengcheng; Breindel, Leonard; Shekhtman, Alexander; Zhang, Qiang (Chemical Science, 2018)

Synthesis and antibacterial studies of teixobactin analogues with non-isostere substitution of enduracididine - Jin, Kang; Po, Kathy Hiu Laam; Kong, Wang Yeuk; Lo, Chung Hei; Lo, Chun Wah; Lam, Ho Yin; Sirinimal, Amaya; Reuven, Jonathan Avraham; Chen, Sheng; Li, Xuechen (Bioorganic & Medicinal Chemistry, 2018)
Keywords: #nosource

Total chemical synthesis of glycocin F and analogues: S -glycosylation confers improved antimicrobial activity - Amso, Zaid; Bisset, Sean W.; Yang, Sung-Hyun; Harris, Paul W. R.; Wright, Tom H.; Navo, Claudio D.; Patchett, Mark L.; Norris, Gillian E.; Brimble, Margaret A. (Chemical Science, 2018)

Studies on the Precise Chemical Synthesis of Human Glycoproteins - Kajihara, Yasuhiro (Bulletin of the Chemical Society of Japan, 2016)
Keywords: #nosource

Synthesis of Poly-Ubiquitin Chains Using a Bifunctional Ubiquitin Monomer - van der Heden van Noort, Gerbrand J.; Kooij, Raymond; Elliott, Paul R.; Komander, David; Ovaa, Huib (Organic Letters, 2017)

Chemical Synthesis of Natural Polyubiquitin Chains through Auxiliary-Mediated Ligation of an Expressed Ubiquitin Isomer - Xu, Ling; Huang, Jian-Feng; Chen, Chen-Chen; Qu, Qian; Shi, Jing; Pan, Man; Li, Yi-Ming (Organic Letters, 2018)
Keywords: #nosource

Total chemical synthesis of human thyroid-stimulating hormone (hTSH) β-subunit: Application of arginine-tagged acetamidomethyl (AcmR) protecting groups - Brailsford, John A.; Stockdill, Jennifer L.; Axelrod, Abram J.; Peterson, Michael T.; Vadola, Paul A.; Johnston, Eric V.; Danishefsky, Samuel J. (Tetrahedron, 2018)

Racemic X-ray structure of L-type calcium channel antagonist Calciseptine prepared by total chemical synthesis - Chen, Chen-Chen; Gao, Shuai; Ai, Hua-Song; Qu, Qian; Tian, Chang-Lin; Li, Yi-Ming (Science China Chemistry, 2018)
Keywords: #nosource

Efficient peptide ligation between allyl-protected Asp and Cys followed by palladium-mediated deprotection - Kamo, Naoki; Hayashi, Gosuke; Okamoto, Akimitsu (Chemical Communications, 2018)
Keywords: #nosource

Ixonnexin from Tick Saliva Promotes Fibrinolysis by Interacting with Plasminogen and Tissue-Type Plasminogen Activator, and Prevents Arterial Thrombosis - Assumpção, Teresa C.; Mizurini, Daniella M.; Ma, Dongying; Monteiro, Robson Q.; Ahlstedt, Sydney; Reyes, Morayma; Kotsyfakis, Michail; Mather, Thomas N.; Andersen, John F.; Lukszo, Jan; Ribeiro, José M. C.; Francischetti, Ivo M. B. (Scientific Reports, 2018)

Amide-forming chemical ligation via O -acyl hydroxamic acids - Dunkelmann, Daniel L.; Hirata, Yuki; Totaro, Kyle A.; Cohen, Daniel T.; Zhang, Chi; Gates, Zachary P.; Pentelute, Bradley L. (Proceedings of the National Academy of Sciences, 2018)

Racemic crystal structures of peptide toxins, GsMTx4 prepared by protein total synthesis - Qu, Qian; Gao, Shuai; Li, Yi-Ming (Journal of Peptide Science, 2018)
Keywords: #nosource

Native chemical ligation at methionine bioisostere norleucine allows for N-terminal chemical protein ligation - Xin, Bo-Tao; van Tol, Bianca D. M.; Ovaa, Huib; Geurink, Paul P. (Organic & Biomolecular Chemistry, 2018)

Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides - Ollivier, Nathalie; Toupy, Thomas; Hartkoorn, Ruben C.; Desmet, Rémi; Monbaliu, Jean-Christophe M.; Melnyk, Oleg (Nature Communications, 2018)

Peptide self-cleavage at a canaline residue: application to a solubilizing tag system for native chemical ligation - Tsuda, Shugo; Nishio, Hideki; Yoshiya, Taku (Chemical Communications, 2018)
Keywords: #nosource

Total synthesis of snake toxin α-bungarotoxin and its analogues by hydrazide-based native chemical ligation - Guo, Xiao-Qi; Liang, Jun; Li, Ying; Zhang, Yong; Huang, Dongliang; Tian, Changlin (Chinese Chemical Letters, 2018)
Keywords: #nosource

Native Chemical Ligation–Photodesulfurization in Flow - Chisholm, Timothy S.; Clayton, Daniel; Dowman, Luke J.; Sayers, Jessica; Payne, Richard J. (Journal of the American Chemical Society, 2018)
Keywords: #nosource

Leveraging the Knorr Pyrazole Synthesis for the Facile Generation of Thioester Surrogates for use in Native Chemical Ligation - Flood, Dillon T.; Hintzen, Jordi C. J.; Bird, Michael J.; Cistrone, Philip A.; Chen, Jason S.; Dawson, Philip E. (Angewandte Chemie International Edition, 2018)

Total synthesis of mambalgin-1/2/3 by two-segment hydrazide-based native chemical ligation: Synthesis of Mambalgin by Hydrazide-based Native Chemical Ligation - Lan, Huan; Wu, Kun; Zheng, Yong; Pan, Man; Huang, Yi-Chao; Gao, Shuai; Zheng, Qing-Yun; Zheng, Ji-Shen; Li, Yi-Ming; Xiao, Bailong; Liu, Lei (Journal of Peptide Science, 2016)
Keywords: #nosource

The discovery of a freezing-induced peptide ligation during the total chemical synthesis of human interferon-ε - Yang, Yin-He; Di, Bin; Yang, Da-Song (Organic & Biomolecular Chemistry, 2018)
Keywords: #nosource

Direct synthesis of N-terminal thiazolidine-containing peptide thioesters from peptide hydrazides - Sato, Kohei; Tanaka, Shoko; Yamamoto, Kazuki; Tashiro, Yosuke; Narumi, Tetsuo; Mase, Nobuyuki (Chemical Communications, 2018)
Keywords: #nosource

Chemical synthesis of a two-domain scorpion toxin LaIT2 and its single-domain analogs to elucidate structural factors important for insecticidal and antimicrobial activities - Juichi, Hironori; Ando, Ryo; Ishido, Takafumi; Miyashita, Masahiro; Nakagawa, Yoshiaki; Miyagawa, Hisashi (Journal of Peptide Science, 2018)
Keywords: #nosource

A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligation - Hou, Wen; Liu, Lei; Zhang, Xiaohong; Liu, Chuanfa (Royal Society Open Science, 2018)

Synthesis of Chlorotoxin by Native Chemical Ligation - Karaca, Ulvi; Kesici, Seçkin; Özçubukçu, Salih (Journal of the Turkish Chemical Society, Section A: Chemistry, 2018)

Mosquito-Derived Anophelin Sulfoproteins Are Potent Antithrombotics - Watson, Emma E.; Liu, Xuyu; Thompson, Robert E.; Ripoll-Rozada, Jorge; Wu, Mike; Alwis, Imala; Gori, Alessandro; Loh, Choy-Theng; Parker, Benjamin L.; Otting, Gottfried; Jackson, Shaun; Pereira, Pedro José Barbosa; et al. (ACS Central Science, 2018)

One-Pot Peptide Ligation–Oxidative Cyclization Protocol for the Preparation of Short-/Medium-Size Disulfide Cyclopeptides - Spengler, Jan; Blanco-Canosa, Juan B.; Forni, Luciano; Albericio, Fernando (Organic Letters, 2018)
Keywords: #nosource

Construction of Challenging Proline–Proline Junctions via Diselenide–Selenoester Ligation Chemistry - Sayers, Jessica; Karpati, Phillip M. T.; Mitchell, Nicholas J.; Goldys, Anna M.; Kwong, Stephen M.; Firth, Neville; Chan, Bun; Payne, Richard J. (Journal of the American Chemical Society, 2018)
Keywords: #nosource

Resin-Bound Crypto-Thioester for Native Chemical Ligation - Naruse, Naoto; Ohkawachi, Kento; Inokuma, Tsubasa; Shigenaga, Akira; Otaka, Akira (Organic Letters, 2018)
Keywords: #nosource

Channel activity of mirror-image M2 proton channel of influenza A virus is blocked by achiral or chiral inhibitors - Guo, Qing-Yan; Zhang, Long-Hua; Zuo, Chao; Huang, Dong-Liang; Wang, Zhipeng A.; Zheng, Ji-Shen; Tian, Chang-Lin (Protein & Cell, 2019)

Synthetic studies toward human interleukin-5 - Liu, Jinrong; Dong, Suwei (Chinese Chemical Letters, 2018)
Keywords: #nosource

Protein Semisynthesis Provides Access to Tau Disease-Associated Post-translational Modifications (PTMs) and Paves the Way to Deciphering the Tau PTM Code in Health and Diseased States - Haj-Yahya, Mahmood; Lashuel, Hilal A. (Journal of the American Chemical Society, 2018)
Keywords: #nosource

De Novo Design To Synthesize Lanthipeptides Involving Cascade Cysteine Reactions: SapB Synthesis as an Example - Chen, Huai; Zhang, Yuan; Li, Qian-Qian; Zhao, Yu-Fen; Chen, Yong-Xiang; Li, Yan-Mei (The Journal of Organic Chemistry, 2018)
Keywords: #nosource

N-terminal Huntingtin (Htt) phosphorylation is a molecular switch regulating Htt aggregation, helical conformation, internalization, and nuclear targeting - DeGuire, Sean M.; Ruggeri, Francesco S.; Fares, Mohamed-Bilal; Chiki, Anass; Cendrowska, Urszula; Dietler, Giovanni; Lashuel, Hilal A. (Journal of Biological Chemistry, 2018)

Enabling N-to-C Ser/Thr Ligation for Convergent Protein Synthesis via Combining Chemical Ligation Approaches - Lee, Chi Lung; Liu, Han; Wong, Clarence T. T.; Chow, Hoi Yee; Li, Xuechen (Journal of the American Chemical Society, 2016)
Keywords: #nosource

Insulin-induced gene 1 (INSIG1) inhibits HIV-1 production by degrading Gag via activity of the ubiquitin ligase TRC8 - Zhang, You; Lu, Jing; Ma, Jing; Liu, Xinqi (Journal of Biological Chemistry, 2019)
Keywords: #nosource

Direct Peptide Cyclization and One-Pot Modification Using the MeDbz Linker - Gless, Bengt H.; Olsen, Christian A. (The Journal of Organic Chemistry, 2018)
Keywords: #nosource

Ubiquitin 7-amino-4-carbamoylmethylcoumarin as an improved fluorogenic substrate for deubiquitinating enzymes - Li, Yi-Tong; Huang, Yi-Chao; Xu, Yang; Pan, Man; Li, Yi-Ming (Tetrahedron, 2016)
Keywords: #nosource

The E15R Point Mutation in Scorpion Toxin Cn2 Uncouples Its Depressant and Excitatory Activities on Human Na V 1.6 - Israel, Mathilde R.; Thongyoo, Panumart; Deuis, Jennifer R.; Craik, David J.; Vetter, Irina; Durek, Thomas (Journal of Medicinal Chemistry, 2018)
Keywords: #nosource

Triple Function of 4-Mercaptophenylacetic Acid Promotes One-Pot Multiple Peptide Ligation - Kamo, Naoki; Hayashi, Gosuke; Okamoto, Akimitsu (Angewandte Chemie International Edition, 2018)
Keywords: #nosource

Total Chemical Synthesis of SUMO and SUMO-Based Probes for Profiling the Activity of SUMO-Specific Proteases - Mulder, Monique P. C.; Merkx, Remco; Witting, Katharina F.; Hameed, Dharjath S.; El Atmioui, Dris; Lelieveld, Lindsey; Liebelt, Frauke; Neefjes, Jacques; Berlin, Ilana; Vertegaal, Alfred C. O.; Ovaa, Huib (Angewandte Chemie International Edition, 2018)

Acceleration of amyloid fibril formation by carboxyl-terminal truncation of human serum amyloid A - Tanaka, Masafumi; Kawakami, Toru; Okino, Nozomi; Sasaki, Kaoru; Nakanishi, Kiwako; Takase, Hiroka; Yamada, Toshiyuki; Mukai, Takahiro (Archives of Biochemistry and Biophysics, 2018)
Keywords: #nosource

A Highly Efficient Synthesis of Polyubiquitin Chains - Qu, Qian; Pan, Man; Gao, Shuai; Zheng, Qing-Yun; Yu, Yuan-Yuan; Su, Jia-Can; Li, Xiang; Hu, Hong-Gang (Advanced Science, 2018)

N, P, and S Codoped Graphene-Like Carbon Nanosheets for Ultrafast Uranium (VI) Capture with High Capacity - Chen, Zhe; Chen, Wanying; Jia, Dashuang; Liu, Yang; Zhang, Anrui; Wen, Tao; Liu, Jian; Ai, Yuejie; Song, Weiguo; Wang, Xiangke (Advanced Science, 2018)

Independent Band Modulation in 2D van der Waals Heterostructures via a Novel Device Architecture - Guo, Zhongxun; Chen, Yan; Zhang, Heng; Wang, Jianlu; Hu, Weida; Ding, Shijin; Zhang, David Wei; Zhou, Peng; Bao, Wenzhong (Advanced Science, 2018)

Self-Sensing Paper Actuators Based on Graphite-Carbon Nanotube Hybrid Films - Amjadi, Morteza; Sitti, Metin (Advanced Science, 2018)

Multimeric Amphipathic α-Helical Sequences for Rapid and Efficient Intracellular Protein Transport at Nanomolar Concentrations - Oh, Jae Hoon; Chong, Seung-Eun; Nam, Sohee; Hyun, Soonsil; Choi, Sejong; Gye, Hyojun; Jang, Sangmok; Jang, Joomyung; Hwang, Sung Won; Yu, Jaehoon; Lee, Yan (Advanced Science, 2018)

Advanced Hierarchical Vesicular Carbon Co-Doped with S, P, N for High-Rate Sodium Storage - Zou, Guoqiang; Hou, Hongshuai; Foster, Christopher W.; Banks, Craig E.; Guo, Tianxiao; Jiang, Yunling; Zhang, Yun; Ji, Xiaobo (Advanced Science, 2018)

Synthesis of neurosecretory protein GM composed of 88 amino acid residues by native chemical ligation - Masuda, Keiko; Furumitsu, Megumi; Ooyama, Haruka; Iwakoshi-Ukena, Eiko; Ukena, Kazuyoshi (Tetrahedron Letters, 2016)
Keywords: #nosource

A synthetic approach to ‘click’ neoglycoprotein analogues of EPO employing one-pot native chemical ligation and CuAAC chemistry - Lee, D. J.; Cameron, A. J.; Wright, T. H.; Harris, P. W. R.; Brimble, M. A. (Chemical Science, 2019)

Synthesis of a Self-Adjuvanting MUC1 Vaccine via Diselenide-Selenoester Ligation-Deselenization - McDonald, David M.; Hanna, Cameron C.; Ashhurst, Anneliese S.; Corcilius, Leo; Byrne, Scott N.; Payne, Richard J. (ACS Chemical Biology, 2018)
Keywords: #nosource

Utility of the Phenacyl Protecting Group in Traceless Protein Semisynthesis through Ligation-Desulfurization Chemistry - Matveenko, Maria; Hackl, Stefanie; Becker, Christian F. W. (ChemistryOpen, 2018)

Generation of the UFM1 Toolkit for Profiling UFM1-Specific Proteases and Ligases - Witting, Katharina F.; van der Heden van Noort, Gerbrand J.; Kofoed, Christian; Talavera Ormeño, Cami; el Atmioui, Dris; Mulder, Monique P. C.; Ovaa, Huib (Angewandte Chemie International Edition, 2018)

Examination of the Deubiquitylation Site Selectivity of USP51 by Using Chemically Synthesized Ubiquitylated Histones - Ai, Huasong; Guo, Yu; Sun, Demeng; Liu, Sanling; Qi, Yunkun; Guo, Jing; Qu, Qian; Gong, Qingyue; Zhao, Suwen; Li, Jiabin; Liu, Lei (ChemBioChem, 2019)
Keywords: #nosource

Preparation of peptide thioesters from naturally occurring sequences using reaction sequence consisting of regioselective S-cyanylation and hydrazinolysis: Preparation of Peptide Thioesters - Miyajima, Rin; Tsuda, Yusuke; Inokuma, Tsubasa; Shigenaga, Akira; Imanishi, Miki; Futaki, Shiroh; Otaka, Akira (Biopolymers, 2016)
Keywords: #nosource

Synthetic Glycoforms Reveal Carbohydrate-Dependent Bioactivity of Human Saposin D - Graf, Christopher G. F.; Schulz, Christian; Schmälzlein, Marina; Heinlein, Christian; Mönnich, Manuel; Perkams, Lukas; Püttner, Markus; Boos, Irene; Hessefort, Markus; Lombana Sanchez, Jose Nelson; Weyand, Michael; Steegborn, Clemens; et al. (Angewandte Chemie International Edition, 2017)
Keywords: #nosource

Anti-Candidal Activity and Functional Mapping of Recombinant and Synthetic Neosartorya fischeri Antifungal Protein 2 (NFAP2) - Tóth, Liliána; Váradi, Györgyi; Borics, Attila; Batta, Gyula; Kele, Zoltán; Vendrinszky, Ákos; Tóth, Roberta; Ficze, Hargita; Tóth, Gábor K.; Vágvölgyi, Csaba; Marx, Florentine; Galgóczy, László (Frontiers in Microbiology, 2018)

Synthesis of selenocysteine-containing cyclic peptides via tandem N -to- S acyl migration and intramolecular selenocysteine-mediated native chemical ligation - Shimodaira, Shingo; Takei, Toshiki; Hojo, Hironobu; Iwaoka, Michio (Chemical Communications, 2018)
Keywords: #nosource

Site-Specific Three-Color Labeling of α-Synuclein via Conjugation to Uniquely Reactive Cysteines during Assembly by Native Chemical Ligation - Lee, Taehyung C.; Moran, Crystal R.; Cistrone, Philip A.; Dawson, Philip E.; Deniz, Ashok A. (Cell Chemical Biology, 2018)

An explorative study towards the chemical synthesis of the immunoglobulin G1 Fc CH3 domain - Grassi, Luigi; Roschger, Cornelia; Stanojlović, Vesna; Cabrele, Chiara (Journal of Peptide Science, 2018)

Toward a Novel Drug To Target the EGF-EGFR Interaction: Design of Metabolically Stable Bicyclic Peptides - Guardiola, Salvador; Seco, Jesús; Varese, Monica; Díaz-Lobo, Mireia; García, Jesús; Teixidó, Meritxell; Nevola, Laura; Giralt, Ernest (ChemBioChem, 2018)

Rapid and low-cost multiplex synthesis of chemokine analogs - Paolini-Bertrand, Marianne; Cerini, Fabrice; Martins, Elsa; Scurci, Ilaria; Hartley, Oliver (Journal of Biological Chemistry, 2018)
Keywords: #nosource

Chemical synthesis of transactivation domain (TAD) of tumor suppressor protein p53 by native chemical ligation of three peptide segments - Baral, Abhishek; Asokan, Aromal; Bauer, Valentin; Kieffer, Bruno; Torbeev, Vladimir (Tetrahedron, 2019)

Facile folding of insulin variants bearing a prosthetic C-peptide prepared by α-ketoacid-hydroxylamine (KAHA) ligation - Boross, Gábor N.; Shimura, Satomi; Besenius, Melissa; Tennagels, Norbert; Rossen, Kai; Wagner, Michael; Bode, Jeffrey W. (Chemical Science, 2018)

Synthesis by native chemical ligation and characterization of the scorpion toxin AmmTx3 - Zoukimian, Claude; Meudal, Hervé; De Waard, Stephan; Ouares, Karima Ait; Nicolas, Sébastien; Canepari, Marco; Béroud, Rémy; Landon, Céline; De Waard, Michel; Boturyn, Didier (Bioorganic & Medicinal Chemistry, 2019)
Keywords: #nosource

Evaluation of Chemical Strategies for Improving the Stability and Oral Toxicity of Insecticidal Peptides - Herzig, Volker; de Araujo, Aline; Greenwood, Kathryn; Chin, Yanni; Windley, Monique; Chong, Youmie; Muttenthaler, Markus; Mobli, Mehdi; Audsley, Neil; Nicholson, Graham; Alewood, Paul; King, Glenn (Biomedicines, 2018)

Cryo-EM structure of the ASIC1a–mambalgin-1 complex reveals that the peptide toxin mambalgin-1 inhibits acid-sensing ion channels through an unusual allosteric effect - Sun, Demeng; Yu, You; Xue, Xiaobin; Pan, Man; Wen, Ming; Li, Siyu; Qu, Qian; Li, Xiaorun; Zhang, Longhua; Li, Xueming; Liu, Lei; Yang, Maojun; et al. (Cell Discovery, 2018)

Using Chemical Synthesis to Probe Structure–Activity Relationships of the Glycoactive Bacteriocin Glycocin F - Bisset, Sean W.; Yang, Sung-Hyun; Amso, Zaid; Harris, Paul W. R.; Patchett, Mark L.; Brimble, Margaret A.; Norris, Gillian E. (ACS Chemical Biology, 2018)
Keywords: #nosource

A bi-terminal protein ligation strategy to probe chromatin structure during DNA damage - Kilic, Sinan; Boichenko, Iuliia; Lechner, Carolin C.; Fierz, Beat (Chemical Science, 2018)

Synthesis of a Double Transmembrane Domain Fragment of Ste2p by Native Chemical Ligation - Balambika, Rema; Inui, Tatsuya; Sargsyan, Hasmik; Arshava, Boris; Cohen, Leah S.; Ding, Fa Xiang; Becker, Jeffrey M.; Naider, Fred (International Journal of Peptide Research and Therapeutics, 2007)
Keywords: #nosource

Synthesis of β-Thiol Phenylalanine for Applications in One-Pot Ligation–Desulfurization Chemistry - Malins, Lara R.; Giltrap, Andrew M.; Dowman, Luke J.; Payne, Richard J. (Organic Letters, 2015)
Keywords: #nosource

Total chemical synthesis by native chemical ligation of the all-D immunoglobulin-like domain 2 of Axl - De Rosa, Lucia; Di Stasi, Rossella; D'Andrea, Luca Domenico (Tetrahedron, 2019)
Keywords: #nosource

A Threonine-Forming Oxazetidine Amino Acid for the Chemical Synthesis of Proteins through KAHA Ligation - Baldauf, Simon; Schauenburg, Dominik; Bode, Jeffrey W. (Angewandte Chemie International Edition, 2019)
Keywords: #nosource

The versatile use of solubilizing trityl tags for difficult peptide/protein synthesis - Tsuda, Shugo; Masuda, Shun; Yoshiya, Taku (Organic & Biomolecular Chemistry, 2019)
Keywords: #nosource

Glycopeptide Synthesis Based on a TFA-Labile Protection Strategy and One-Pot Four-Segment Ligation for the Synthesis of O-Glycosylated Histone H2A: Glycopeptide Synthesis Based on a TFA-Labile Protection Strategy and One-Pot Four-Segment Ligation for the Synthesis of O-Glycosylated Histone H2A - Asahina, Yuya; Kawakami, Toru; Hojo, Hironobu (European Journal of Organic Chemistry, 2019)
Keywords: #nosource

A chemical approach for the synthesis of the DNA-binding domain of the oncoprotein MYC - Calo-Lapido, Renata; Penas, Cristina; Jiménez-Balsa, Adrián; Vázquez, M. Eugenio; Mascareñas, José L. (Organic & Biomolecular Chemistry, 2019)
Keywords: #nosource

α-Selective Lysine Ligation and Application in Chemical Synthesis of Interferon Gamma - Dao, Yuankun; Han, Lin; Wang, Hanxuan; Dong, Suwei (Organic Letters, 2019)

VHH characterization. Comparison of recombinant with chemically synthesized anti‐HER2 VHH - Hartmann, Lucie; Botzanowski, Thomas; Galibert, Mathieu; Jullian, Magali; Chabrol, Eric; Zeder‐Lutz, Gabrielle; Kugler, Valérie; Stojko, Johann; Strub, Jean‐Marc; Ferry, Gilles; Frankiewicz, Lukasz; Puget, Karine; et al. (Protein Science, 2019)
Keywords: #nosource

Rapid assembly and profiling of an anticoagulant sulfoprotein library - Watson, Emma E.; Ripoll-Rozada, Jorge; Lee, Ashley C.; Wu, Mike C. L.; Franck, Charlotte; Pasch, Tim; Premdjee, Bhavesh; Sayers, Jessica; Pinto, Maria F.; Martins, Pedro M.; Jackson, Shaun P.; Pereira, Pedro José Barbosa; et al. (Proceedings of the National Academy of Sciences, 2019)
Keywords: #nosource

Synthesis of tumor necrosis factor α for use as a mirror-image phage display target - Petersen, Mark E.; Jacobsen, Michael T.; Kay, Michael S. (Organic & Biomolecular Chemistry, 2016)

Copying Life: Synthesis of an Enzymatically Active Mirror-Image DNA-Ligase Made of D-Amino Acids - Weidmann, Joachim; Schnölzer, Martina; Dawson, Philip E.; Hoheisel, Jörg D. (Cell Chemical Biology, 2019)
Keywords: #nosource

Copper-Mediated Selenazolidine Deprotection Enables One-Pot Chemical Synthesis of Challenging Proteins - Zhao, Zhenguang; Metanis, Norman (Angewandte Chemie International Edition, 2019)
Keywords: #nosource

Ligation of Soluble but Unreactive Peptide Segments in the Chemical Synthesis of Haemophilus Influenzae DNA Ligase - Zhang, Baochang; Deng, Qiang; Zuo, Chong; Yan, Bingjia; Zuo, Chao; Cao, Xiu-Xiu; Zhu, Ting F.; Zheng, Ji-Shen; Liu, Lei (Angewandte Chemie International Edition, 2019)
Keywords: #nosource

Development of Powerful Auxiliary-Mediated Ligation To Facilitate Rapid Protein Assembly - Yin, Hongli; Lu, Dan; Wang, Siyao; Wang, Ping (Organic Letters, 2019)
Keywords: #nosource

Cysteinylprolyl imide (CPI) peptide: a highly reactive and easily accessible crypto-thioester for chemical protein synthesis - Yanase, Masafumi; Nakatsu, Koki; Cardos, Charlane Joy; Konda, Yoshiki; Hayashi, Gosuke; Okamoto, Akimitsu (Chemical Science, 2019)

Chemoselective modifications for the traceless ligation of thioamide-containing peptides and proteins - Wang, Yanxin J.; Szantai-Kis, D. Miklos; Petersson, E. James (Organic & Biomolecular Chemistry, 2016)

Total synthesis of TRADD death domain with arginine N-GlcNAcylation by hydrazide-based native chemical ligation - Wu, Ye; Li, Yulei; Cong, Wei; Zou, Yan; Li, Xiang; Hu, Honggang (Chinese Chemical Letters, 2019)
Keywords: #nosource

Exploring the Functional Consequences of Protein Backbone Alteration in Ubiquitin through Native Chemical Ligation - Werner, Halina M.; Estabrooks, Samuel K.; Preston, G. Michael; Brodsky, Jeffrey L.; Horne, W. Seth (ChemBioChem, 2019)
Keywords: #nosource

Chimeric protein probes for C5a receptors through fusion of the anaphylatoxin C5a core region with a small-molecule antagonist - Zuo, Chao; Shi, Wei-Wei; Chen, Xiao-Xu; Glatz, Marie; Riedl, Bernd; Flamme, Ingo; Pook, Elisabeth; Wang, Jiawei; Fang, Ge-Min; Bierer, Donald; Liu, Lei (Science China Chemistry, 2019)
Keywords: #nosource

Development of a dual-functional conjugate of antigenic peptide and Fc-III mimetics (DCAF) for targeted antibody blocking - Zhang, Lin; Shen, Hao; Gong, Yiyi; Pang, Xiaojing; Yi, Meiqi; Guo, Lin; Li, Jin; Arroyo, Sam; Lu, Xin; Ovchinnikov, Sergey; Cheng, Gong; Liu, Xudong; et al. (Chemical Science, 2019)

Examining Several Strategies for the Chemical Synthesis of Phosphorylated Histone H3 Reveals the Effectiveness of the Convergent Approach: Examining Several Strategies for the Chemical Synthesis of Phosphorylated Histone H3 Reveals the Effectiveness of the Convergent Approach - Jbara, Muhammad; Maity, Suman Kumar; Brik, Ashraf (European Journal of Organic Chemistry, 2019)
Keywords: #nosource

Chemical Synthesis of Atomically Tailored SUMO E2 Conjugating Enzymes for the Formation of Covalently Linked SUMO–E2–E3 Ligase Ternary Complexes - Zhang, Yinfeng; Hirota, Tsuyoshi; Kuwata, Keiko; Oishi, Shunsuke; Gramani, Subramanian G.; Bode, Jeffrey W. (Journal of the American Chemical Society, 2019)
Keywords: #nosource

Solubilizing Trityl‐Type Tag To Synthesize Asx/Glx‐Containing Peptides - Tsuda, Shugo; Masuda, Shun; Yoshiya, Taku (ChemBioChem, 2019)
Keywords: #nosource

Development of Mirror-Image Screening Systems for XIAP BIR3 Domain Inhibitors - Shu, Keitou; Iwamoto, Naoya; Honda, Kaori; Kondoh, Yasumitsu; Hirano, Hiroyuki; Osada, Hiroyuki; Ohno, Hiroaki; Fujii, Nobutaka; Oishi, Shinya (Bioconjugate Chemistry, 2019)
Keywords: #nosource

Native Chemical Ligation to Minimize Aspartimide Formation during Chemical Synthesis of Small LDLa Protein - Tailhades, Julien; Sethi, Ashish; Petrie, Emma J.; Gooley, Paul R.; Bathgate, Ross A.; Wade, John D.; Hossain, Mohammed A. (Chemistry - A European Journal, 2016)
Keywords: #nosource

Palladium-Mediated Cleavage of Proteins with Thiazolidine-Modified Backbone in Live Cells - Mann, Guy; Satish, Gandhesiri; Meledin, Roman; Vamisetti, Ganga B.; Brik, Ashraf (Angewandte Chemie International Edition, 2019)
Keywords: #nosource

Use of Serine/Threonine Ligation for the Total Chemical Synthesis of HMGA1a Protein with Site‐Specific Lysine Acetylations - Liu, Heng; Liu, Han; Li, Xuechen (ChemPlusChem, 2019)
Keywords: #nosource

Chemical synthesis of the ubiquitinated form of histone H3 and its effect on DNA methyltransferase 1 - Kawakami, Toru; Mishima, Yuichi; Takazawa, Masaya; Hojo, Hironobu; Suetake, Isao (Journal of Peptide Science, 2019)
Keywords: #nosource

Photo-cleavable purification/protection handle assisted synthesis of giant modified proteins with tandem repeats - Liu, Xueyi; Liu, Jiazhi; Wu, Zhichao; Chen, Liangbiao; Wang, Siyao; Wang, Ping (Chemical Science, 2019)

Palladium-Mediated Direct Disulfide Bond Formation in Proteins Containing S-Acetamidomethyl-cysteine under Aqueous Conditions - Laps, Shay; Sun, Hao; Kamnesky, Guy; Brik, Ashraf (Angewandte Chemie International Edition, 2019)
Keywords: #nosource

Diselenide–selenoester ligation for chemical protein synthesis - Kulkarni, Sameer S.; Watson, Emma E.; Premdjee, Bhavesh; Conde-Frieboes, Kilian W.; Payne, Richard J. (Nature Protocols, 2019)
Keywords: #nosource

Convergent Hybrid Phase Ligation Strategy for Efficient Total Synthesis of Large Proteins Demonstrated for 212-residue Linker Histone H1.2 - Hong, Ziyong Z.; Yu, Ruixuan R.; Zhang, Xiaoyu; Webb, Allison M.; Burge, Nathaniel L.; Poirier, Michael G.; Ottesen, Jennifer J. (bioRxiv, 2019)
Simple and efficient total chemical synthesis of large proteins remains a significant challenge. Here, we report development of a convergent hybrid phase native chemical ligation (CHP-NCL) strategy that should be generally applicable for facile preparation of large proteins. Key to the strategy is the use of sequential ligation on the solid phase for the directed assembly of ~100-residue segments from short, synthetically accessible peptide components. These segments can then be assembled via convergent solution phase ligation, exploiting o-aminoaniline as a chemically flexible cryptic thioester with multiple activation modalitiies on resin and . We demonstrate the feasibility of our approach through the total synthesis of 212-residue linker histone H1.2 in unmodified, phosphorylated, and citrullinated forms, each from eight component peptide segments. We further demonstrate that fully synthetic H1.2 replicates the binding interactions of linker histones to intact mononucleosomes, as a proxy for the essential function of linker histones in the formation and regulation of higher order chromatin structure.

Chemoselective derivitization of folded synthetic insulin variants with potassium acyltrifluoroborates (KATs) - Boross, Gabor N.; Schauenburg, Dominik; Bode, Jeffrey W. (Helvetica Chimica Acta, 2018)
Keywords: #nosource

Single Posttranslational Modifications in the Central Repeat Domains of Tau4 Impact its Aggregation and Tubulin Binding - Ellmer, Doris; Brehs, Manuel; Haj-Yahya, Mahmood; Lashuel, Hilal A.; Becker, Christian F. W. (Angewandte Chemie International Edition, 2019)

t-boc synthesis of huwentoxin-i through native chemical ligation incorporating a trifluoromethanesulfonic acid cleavage strategy: t-Boc Synthesis of Huwentoxin-I - Thapa, Parashar; Cabalteja, Chino C.; Philips, Edwin E.; Espiritu, Michael J.; Peigneur, Steve; Mille, Bea G.; Tytgat, Jan; Cummins, Theodore R.; Bingham, Jon-Paul (Biopolymers, 2016)

Auxiliary-assisted chemical ubiquitylation of NEMO and linear extension by HOIP - Burlina, Fabienne; Abdel-Aal, Abu-Baker M.; Raz, Richard; Pinzuti, Irene; Papageorgiou, George; Li, Jiejin; Antrobus, Robin; Martin, Stephen R.; Kunzelmann, Simone; Stieglitz, Benjamin; Offer, John (Communications Chemistry, 2019)

Development of a sufficiently reactive thioalkylester involving the side-chain thiol of cysteine applicable for kinetically controlled ligation: Development of a Sufficiently Reactive Thioalkylester - Tsuda, Shugo; Mochizuki, Masayoshi; Nishio, Hideki; Yoshiya, Taku; Nishiuchi, Yuji (Biopolymers, 2016)
Keywords: #nosource

Combination of Thiol-Additive-Free Native Chemical Ligation/Desulfurization and Intentional Replacement of Alanine with Cysteine - Tsuda, Shugo; Mochizuki, Masayoshi; Nishio, Hideki; Yoshiya, Taku (ChemBioChem, 2016)
Keywords: #nosource

A synthetic molecular system capable of mirror-image genetic replication and transcription - Wang, Zimou; Xu, Weiliang; Liu, Lei; Zhu, Ting F. (Nature Chemistry, 2016)
Keywords: #nosource

Chemical Synthesis of K48-Linked Diubiquitin by Incorporation of a Lysine-Linked Auxiliary Handle - Xie, Rui-Long; Xu, Ling; Li, Jia-Bin; Chu, Guo-Chao; Wang, Tao; Huang, Yi-Chao; Li, Yi-Ming (European Journal of Organic Chemistry, 2016)
Keywords: #nosource

Efficient chemical synthesis for the analogue of ubiquitin-based probe Ub–AMC with native bioactivity - Xu, Ling; Xu, Yang; Qu, Qian; Guan, Chao-Jian; Chu, Guo-Chao; Shi, Jing; Li, Yi-Ming (RSC Advances, 2016)
Keywords: #nosource

Total chemical synthesis of bivalently modified H3 by improved three-segment native chemical ligation - Zheng, Yong; Wu, Fangming; Ling, Shenglong; Li, Jia-Bin; Tian, Changlin (Chinese Chemical Letters, 2019)
Keywords: #nosource

The Role of the Conserved SUMO-2/3 Cysteine Residue on Domain Structure Investigated Using Protein Chemical Synthesis - Bouchenna, Jennifer; Sénéchal, Magalie; Drobecq, Hervé; Stankovic-Valentin, Nicolas; Vicogne, Jérôme; Melnyk, Oleg (Bioconjugate Chemistry, 2019)
Keywords: #nosource

Chemical Synthesis of Cys-Containing Protein via Chemoselective Deprotection with Different Palladium Complexes - Kamo, Naoki; Hayashi, Gosuke; Okamoto, Akimitsu (Organic Letters, 2019)
Keywords: #nosource

Protein Chemical Synthesis Combined with Mirror‐Image Phage Display Yields d ‐Peptide EGF Ligands that Block the EGF–EGFR Interaction - Díaz‐Perlas, Cristina; Varese, Monica; Guardiola, Salvador; Sánchez‐Navarro, Macarena; García, Jesús; Teixidó, Meritxell; Giralt, Ernest (ChemBioChem, 2019)
Keywords: #nosource

Diverse fate of ubiquitin chain moieties: The proximal is degraded with the target, and the distal protects the proximal from removal and recycles - Sun, Hao; Mali, Sachitanand M.; Singh, Sumeet K.; Meledin, Roman; Brik, Ashraf; Kwon, Yong Tae; Kravtsova-Ivantsiv, Yelena; Bercovich, Beatrice; Ciechanover, Aaron (Proceedings of the National Academy of Sciences, 2019)
Keywords: #nosource

A Modular Ligation Strategy for Asymmetric Bivalent Nucleosomes Trimethylated at K36 and K27 - Guidotti, Nora; Lechner, Carolin C.; Bachmann, Andreas L.; Fierz, Beat (ChemBioChem, 2019)
Keywords: #nosource

Semisynthesis of unnatural amino acid mutants of paxillin: Protein probes for cell migration studies - Vogel, Elizabeth M.; Imperiali, Barbara (Protein Science, 2007)

Semisynthesis of unnatural amino acid mutants of paxillin: Protein probes for cell migration studies - Vogel, Elizabeth M.; Imperiali, Barbara (Protein Science, 2007)

HF-Free Boc Synthesis of Peptide Thioesters for Ligation and Cyclization - Raz, Richard; Burlina, Fabienne; Ismail, Mohamed; Downward, Julian; Li, Jiejin; Smerdon, Stephen J.; Quibell, Martin; White, Peter D.; Offer, John (Angewandte Chemie International Edition, 2016)

HF-Free Boc Synthesis of Peptide Thioesters for Ligation and Cyclization - Raz, Richard; Burlina, Fabienne; Ismail, Mohamed; Downward, Julian; Li, Jiejin; Smerdon, Stephen J.; Quibell, Martin; White, Peter D.; Offer, John (Angewandte Chemie International Edition, 2016)

Diverse fate of ubiquitin chain moieties: The proximal is degraded with the target, and the distal protects the proximal from removal and recycles - Sun, Hao; Mali, Sachitanand M.; Singh, Sumeet K.; Meledin, Roman; Brik, Ashraf; Kwon, Yong Tae; Kravtsova-Ivantsiv, Yelena; Bercovich, Beatrice; Ciechanover, Aaron (Proceedings of the National Academy of Sciences, 2019)
One of the enigmas in the ubiquitin (Ub) field is the requirement for a poly-Ub chain as a proteasomal targeting signal. The canonical chain appears to be longer than the distance between the two Ub-binding proteasomal receptors. Furthermore, genetic manipulation has shown that one receptor subunit is sufficient, which suggests that a single Ub can serve as a degradation signal. To shed light on this mystery, we chemically synthesized tetra-Ub, di-Ub (K 48 -based), and mono-Ub adducts of HA-α-globin, where the distal or proximal Ub moieties were tagged differentially with either Myc or Flag. When incubated in a crude cell extract, the distal Ub moiety in the tetra-Ub adduct was mostly removed by deubiquitinating enzymes (DUBs) and reconjugated to other substrates in the extract. In contrast, the proximal moiety was most likely degraded with the substrate. The efficacy of degradation was proportionate to the chain length; while tetra-Ub globin was an efficient substrate, with mono-Ub globin, we observed rapid removal of the Ub moiety with almost no degradation of the free globin. Taken together, these findings suggest that the proximal moieties are necessary for securing the association of the substrate with the proteasome along the proteolytic process, whereas the distal moieties are important in protecting the proximal moieties from premature deubiquitination. Interestingly, when the same experiment was carried out using purified 26S proteasome, mono- and tetra-Ub globin were similarly degraded, highlighting the roles of the entire repertoire of cellular DUBs in regulating the degradation of proteasomal substrates.

Diverse fate of ubiquitin chain moieties: The proximal is degraded with the target, and the distal protects the proximal from removal and recycles - Sun, Hao; Mali, Sachitanand M.; Singh, Sumeet K.; Meledin, Roman; Brik, Ashraf; Kwon, Yong Tae; Kravtsova-Ivantsiv, Yelena; Bercovich, Beatrice; Ciechanover, Aaron (Proceedings of the National Academy of Sciences, 2019)
One of the enigmas in the ubiquitin (Ub) field is the requirement for a poly-Ub chain as a proteasomal targeting signal. The canonical chain appears to be longer than the distance between the two Ub-binding proteasomal receptors. Furthermore, genetic manipulation has shown that one receptor subunit is sufficient, which suggests that a single Ub can serve as a degradation signal. To shed light on this mystery, we chemically synthesized tetra-Ub, di-Ub (K 48 -based), and mono-Ub adducts of HA-α-globin, where the distal or proximal Ub moieties were tagged differentially with either Myc or Flag. When incubated in a crude cell extract, the distal Ub moiety in the tetra-Ub adduct was mostly removed by deubiquitinating enzymes (DUBs) and reconjugated to other substrates in the extract. In contrast, the proximal moiety was most likely degraded with the substrate. The efficacy of degradation was proportionate to the chain length; while tetra-Ub globin was an efficient substrate, with mono-Ub globin, we observed rapid removal of the Ub moiety with almost no degradation of the free globin. Taken together, these findings suggest that the proximal moieties are necessary for securing the association of the substrate with the proteasome along the proteolytic process, whereas the distal moieties are important in protecting the proximal moieties from premature deubiquitination. Interestingly, when the same experiment was carried out using purified 26S proteasome, mono- and tetra-Ub globin were similarly degraded, highlighting the roles of the entire repertoire of cellular DUBs in regulating the degradation of proteasomal substrates.

Selenolysine: A New Tool for Traceless Isopeptide Bond Formation - Dardashti, Rebecca Notis; Kumar, Shailesh; Sternisha, Shawn M.; Reddy, Post Sai; Miller, Brian G.; Metanis, Norman (Chemistry – A European Journal, 2020)

Selenolysine: A New Tool for Traceless Isopeptide Bond Formation - Dardashti, Rebecca Notis; Kumar, Shailesh; Sternisha, Shawn M.; Reddy, Post Sai; Miller, Brian G.; Metanis, Norman (Chemistry – A European Journal, 2020)

Chemical Synthesis of Diubiquitin-Based Photoaffinity Probes for Selectively Profiling Ubiquitin-Binding Proteins - Liang, Jun; Zhang, Lin; Tan, Xiang-Long; Qi, Yun-Kun; Feng, Shan; Deng, Haiteng; Yan, Yijing; Zheng, Ji-Shen; Liu, Lei; Tian, Chang-Lin (Angewandte Chemie International Edition, 2017)

Chemical Synthesis of Diubiquitin-Based Photoaffinity Probes for Selectively Profiling Ubiquitin-Binding Proteins - Liang, Jun; Zhang, Lin; Tan, Xiang-Long; Qi, Yun-Kun; Feng, Shan; Deng, Haiteng; Yan, Yijing; Zheng, Ji-Shen; Liu, Lei; Tian, Chang-Lin (Angewandte Chemie International Edition, 2017)