Chemical Protein Synthesis by Solid Phase Ligation of Unprotected Peptide Segments - Canne, Lynne E.; Botti, Paolo; Simon, Reyna J.; Chen, Yijun; Dennis, Edward A.; Kent, Stephen B. H. (Journal of the American Chemical Society, 1999)

Development of Mirror-Image Screening Systems for XIAP BIR3 Domain Inhibitors - Shu, Keitou; Iwamoto, Naoya; Honda, Kaori; Kondoh, Yasumitsu; Hirano, Hiroyuki; Osada, Hiroyuki; Ohno, Hiroaki; Fujii, Nobutaka; Oishi, Shinya (Bioconjugate Chemistry, 2019)

Total synthesis of TRADD death domain with arginine N-GlcNAcylation by hydrazide-based native chemical ligation - Wu, Ye; Li, Yulei; Cong, Wei; Zou, Yan; Li, Xiang; Hu, Honggang (Chinese Chemical Letters, 2020)

Total chemical synthesis of the site-selective azide-labeled [I66A]HIV-1 protease - Qi, Yun-Kun; Chang, Hao-Nan; Pan, Kai-Mai; Tian, Chang-Lin; Zheng, Ji-Shen (Chemical Communications, 2015)
The first total chemical synthesis of the site-selective azide-labeled [I66A]HIV-1 protease is described by native chemical ligation. , The first total chemical synthesis of the site-selective azide-labeled [I66A]HIV-1 protease is described by native chemical ligation. Chemical synthesis of azide-labeled proteins would provide useful protein tools for biochemical, biophysical or medical studies.

Total chemical synthesis of an Orf virus protein, ORFV002, an inhibitor of the master gene regulator NF-κB: Chemical Synthesis of ORFV002 - Son, S. J.; Harris, P. W. R.; Squire, C. J.; Baker, E. N.; Kent, S. B. H.; Brimble, M. A. (Biopolymers, 2014)

Expedient Total Synthesis of Small to Medium-Sized Membrane Proteins via Fmoc Chemistry - Zheng, Ji-Shen; Yu, Mu; Qi, Yun-Kun; Tang, Shan; Shen, Fei; Wang, Zhi-Peng; Xiao, Liang; Zhang, Longhua; Tian, Chang-Lin; Liu, Lei (Journal of the American Chemical Society, 2014)

Chemical Synthesis and Expression of the HIV-1 Rev Protein - Siman, Peter; Blatt, Ofrah; Moyal, Tal; Danieli, Tsafi; Lebendiker, Mario; Lashuel, Hilal A.; Friedler, Assaf; Brik, Ashraf (ChemBioChem, 2011)

Synthetic studies toward human interleukin-5 - Liu, Jinrong; Dong, Suwei (Chinese Chemical Letters, 2018)

Development of Mirror-Image Screening Systems for XIAP BIR3 Domain Inhibitors - Shu, Keitou; Iwamoto, Naoya; Honda, Kaori; Kondoh, Yasumitsu; Hirano, Hiroyuki; Osada, Hiroyuki; Ohno, Hiroaki; Fujii, Nobutaka; Oishi, Shinya (Bioconjugate Chemistry, 2019)

Copper‐Mediated Selenazolidine Deprotection Enables One‐Pot Chemical Synthesis of Challenging Proteins - Zhao, Zhenguang; Metanis, Norman (Angewandte Chemie International Edition, 2019)

Total chemical synthesis of a membrane protein domain analogue containing two transmembrane helices: functional reconstitution of the semisynthetic sensory rhodopsin/transducer complex: SEMISYNTHESIS OF A PHOTORECEPTOR MEMBRANE COMPLEX - Dittmann, Marc; Seidel, Ralf; Chizhov, Igor; Engelhard, Martin (Journal of Peptide Science, 2014)

Total chemical synthesis of the site-selective azide-labeled [I66A]HIV-1 protease - Qi, Yun-Kun; Chang, Hao-Nan; Pan, Kai-Mai; Tian, Chang-Lin; Zheng, Ji-Shen (Chemical Communications, 2015)
The first total chemical synthesis of the site-selective azide-labeled [I66A]HIV-1 protease is described by native chemical ligation. , The first total chemical synthesis of the site-selective azide-labeled [I66A]HIV-1 protease is described by native chemical ligation. Chemical synthesis of azide-labeled proteins would provide useful protein tools for biochemical, biophysical or medical studies.

Chemical Synthesis of the 12 kDa Human Myokine Irisin by α -Ketoacid-Hydroxylamine (KAHA) Ligation - Wucherpfennig, Thomas G.; Müller, Sebastian; Wolfrum, Christian; Bode, Jeffrey W. (Helvetica Chimica Acta, 2016)

Expedient Total Synthesis of Small to Medium-Sized Membrane Proteins via Fmoc Chemistry - Zheng, Ji-Shen; Yu, Mu; Qi, Yun-Kun; Tang, Shan; Shen, Fei; Wang, Zhi-Peng; Xiao, Liang; Zhang, Longhua; Tian, Chang-Lin; Liu, Lei (Journal of the American Chemical Society, 2014)

Total chemical synthesis of human thyroid-stimulating hormone (hTSH) β-subunit: Application of arginine-tagged acetamidomethyl (AcmR) protecting groups - Brailsford, John A.; Stockdill, Jennifer L.; Axelrod, Abram J.; Peterson, Michael T.; Vadola, Paul A.; Johnston, Eric V.; Danishefsky, Samuel J. (Tetrahedron, 2018)

Synthetic studies toward human interleukin-5 - Liu, Jinrong; Dong, Suwei (Chinese Chemical Letters, 2018)

Copper‐Mediated Selenazolidine Deprotection Enables One‐Pot Chemical Synthesis of Challenging Proteins - Zhao, Zhenguang; Metanis, Norman (Angewandte Chemie International Edition, 2019)

The Role of the Conserved SUMO-2/3 Cysteine Residue on Domain Structure Investigated Using Protein Chemical Synthesis - Bouchenna, Jennifer; Sénéchal, Magalie; Drobecq, Hervé; Stankovic-Valentin, Nicolas; Vicogne, Jérôme; Melnyk, Oleg (Bioconjugate Chemistry, 2019)

Total chemical synthesis of a membrane protein domain analogue containing two transmembrane helices: functional reconstitution of the semisynthetic sensory rhodopsin/transducer complex: SEMISYNTHESIS OF A PHOTORECEPTOR MEMBRANE COMPLEX - Dittmann, Marc; Seidel, Ralf; Chizhov, Igor; Engelhard, Martin (Journal of Peptide Science, 2014)

Probing the Frontiers of Glycoprotein Synthesis: The Fully Elaborated β-Subunit of the Human Follicle-Stimulating Hormone - Nagorny, Pavel; Sane, Neeraj; Fasching, Bernhard; Aussedat, Baptiste; Danishefsky, Samuel J. (Angewandte Chemie, 2012)

Chemical Synthesis of the 12 kDa Human Myokine Irisin by α -Ketoacid-Hydroxylamine (KAHA) Ligation - Wucherpfennig, Thomas G.; Müller, Sebastian; Wolfrum, Christian; Bode, Jeffrey W. (Helvetica Chimica Acta, 2016)

An Efficient One-Pot Four-Segment Condensation Method for Protein Chemical Synthesis - Tang, Shan; Si, Yan-Yan; Wang, Zhi-Peng; Mei, Kun-Rong; Chen, Xin; Cheng, Jing-Yuan; Zheng, Ji-Shen; Liu, Lei (Angewandte Chemie International Edition, 2015)

Total chemical synthesis of human thyroid-stimulating hormone (hTSH) β-subunit: Application of arginine-tagged acetamidomethyl (AcmR) protecting groups - Brailsford, John A.; Stockdill, Jennifer L.; Axelrod, Abram J.; Peterson, Michael T.; Vadola, Paul A.; Johnston, Eric V.; Danishefsky, Samuel J. (Tetrahedron, 2018)

Modulation of Reactivity in Native Chemical Ligation through the Use of Thiol Additives - Dawson, Philip E.; Churchill, Michael J.; Ghadiri, M. Reza; Kent, Stephen B. H. (Journal of the American Chemical Society, 1997)

Synthesis of Peptides and Proteins without Cysteine Residues by Native Chemical Ligation Combined with Desulfurization - Yan, Liang Z.; Dawson, Philip E. (Journal of the American Chemical Society, 2001)

Robust Chemical Synthesis of Membrane Proteins through a General Method of Removable Backbone Modification - Zheng, Ji-Shen; He, Yao; Zuo, Chao; Cai, Xiao-Ying; Tang, Shan; Wang, Zhipeng A.; Zhang, Long-Hua; Tian, Chang-Lin; Liu, Lei (Journal of the American Chemical Society, 2016)

Interrogation of MDM2 Phosphorylation in p53 Activation Using Native Chemical Ligation: The Functional Role of Ser17 Phosphorylation in MDM2 Reexamined - Zhan, Changyou; Varney, Kristen; Yuan, Weirong; Zhao, Le; Lu, Wuyuan (Journal of the American Chemical Society, 2012)

The Role of the Conserved SUMO-2/3 Cysteine Residue on Domain Structure Investigated Using Protein Chemical Synthesis - Bouchenna, Jennifer; Sénéchal, Magalie; Drobecq, Hervé; Stankovic-Valentin, Nicolas; Vicogne, Jérôme; Melnyk, Oleg (Bioconjugate Chemistry, 2019)

Probing the Frontiers of Glycoprotein Synthesis: The Fully Elaborated β-Subunit of the Human Follicle-Stimulating Hormone - Nagorny, Pavel; Sane, Neeraj; Fasching, Bernhard; Aussedat, Baptiste; Danishefsky, Samuel J. (Angewandte Chemie, 2012)

An Efficient One-Pot Four-Segment Condensation Method for Protein Chemical Synthesis - Tang, Shan; Si, Yan-Yan; Wang, Zhi-Peng; Mei, Kun-Rong; Chen, Xin; Cheng, Jing-Yuan; Zheng, Ji-Shen; Liu, Lei (Angewandte Chemie International Edition, 2015)

Modulation of Reactivity in Native Chemical Ligation through the Use of Thiol Additives - Dawson, Philip E.; Churchill, Michael J.; Ghadiri, M. Reza; Kent, Stephen B. H. (Journal of the American Chemical Society, 1997)

Synthesis of Peptides and Proteins without Cysteine Residues by Native Chemical Ligation Combined with Desulfurization - Yan, Liang Z.; Dawson, Philip E. (Journal of the American Chemical Society, 2001)

Palladium‐Mediated Direct Disulfide Bond Formation in Proteins Containing S‐Acetamidomethyl‐cysteine under Aqueous Conditions - Laps, Shay; Sun, Hao; Kamnesky, Guy; Brik, Ashraf (Angewandte Chemie International Edition, 2019)

Robust Chemical Synthesis of Membrane Proteins through a General Method of Removable Backbone Modification - Zheng, Ji-Shen; He, Yao; Zuo, Chao; Cai, Xiao-Ying; Tang, Shan; Wang, Zhipeng A.; Zhang, Long-Hua; Tian, Chang-Lin; Liu, Lei (Journal of the American Chemical Society, 2016)

Functional Interrogation of the N-Terminal Lid of MDMX in p53 Binding via Native Chemical Ligation - Chen, Xishan; Lu, Weiyue (CHEMICAL & PHARMACEUTICAL BULLETIN, 2016)

Interrogation of MDM2 Phosphorylation in p53 Activation Using Native Chemical Ligation: The Functional Role of Ser17 Phosphorylation in MDM2 Reexamined - Zhan, Changyou; Varney, Kristen; Yuan, Weirong; Zhao, Le; Lu, Wuyuan (Journal of the American Chemical Society, 2012)

Palladium‐Mediated Direct Disulfide Bond Formation in Proteins Containing S‐Acetamidomethyl‐cysteine under Aqueous Conditions - Laps, Shay; Sun, Hao; Kamnesky, Guy; Brik, Ashraf (Angewandte Chemie International Edition, 2019)

Functional Interrogation of the N-Terminal Lid of MDMX in p53 Binding via Native Chemical Ligation - Chen, Xishan; Lu, Weiyue (CHEMICAL & PHARMACEUTICAL BULLETIN, 2016)

Total chemical synthesis, refolding, and crystallographic structure of fully active immunophilin calstabin 2 (FKBP12.6): Chemical Synthesis of Catalytically Active Calstabin and Mutants - Bacchi, Marine; Jullian, Magali; Sirigu, Serena; Fould, Benjamin; Huet, Tiphaine; Bruyand, Lisa; Antoine, Mathias; Vuillard, Laurent; Ronga, Luisa; Chavas, Leonard M. G.; Nosjean, Olivier; Ferry, Gilles; et al. (Protein Science, 2016)

Synthesis of the Src SH2 domain and its application in bioassays for mirror-image screening - Shu, Keitou; Noguchi, Taro; Honda, Kaori; Kondoh, Yasumitsu; Osada, Hiroyuki; Ohno, Hiroaki; Fujii, Nobutaka; Oishi, Shinya (RSC Advances, 2017)
Mirror-image screening systems for Src SH2 domain inhibitors were established using a synthetic Src SH2 domain. , The Src SH2 domain was synthesized via native chemical ligation of two fragment peptides. The facile protocol was used to prepare the mirror-image SH2 domain ( d -Src SH2 domain) and tetramethylrhodamine-labeled SH2 domains. The synthesized SH2 domains were correctly folded and showed activity, and using these proteins we established bioassays to identify novel Src SH2 domain inhibitors from an unexplored mirror-image library of natural products.

Total chemical synthesis, refolding, and crystallographic structure of fully active immunophilin calstabin 2 (FKBP12.6): Chemical Synthesis of Catalytically Active Calstabin and Mutants - Bacchi, Marine; Jullian, Magali; Sirigu, Serena; Fould, Benjamin; Huet, Tiphaine; Bruyand, Lisa; Antoine, Mathias; Vuillard, Laurent; Ronga, Luisa; Chavas, Leonard M. G.; Nosjean, Olivier; Ferry, Gilles; et al. (Protein Science, 2016)

Synthesis of the Src SH2 domain and its application in bioassays for mirror-image screening - Shu, Keitou; Noguchi, Taro; Honda, Kaori; Kondoh, Yasumitsu; Osada, Hiroyuki; Ohno, Hiroaki; Fujii, Nobutaka; Oishi, Shinya (RSC Advances, 2017)
Mirror-image screening systems for Src SH2 domain inhibitors were established using a synthetic Src SH2 domain. , The Src SH2 domain was synthesized via native chemical ligation of two fragment peptides. The facile protocol was used to prepare the mirror-image SH2 domain ( d -Src SH2 domain) and tetramethylrhodamine-labeled SH2 domains. The synthesized SH2 domains were correctly folded and showed activity, and using these proteins we established bioassays to identify novel Src SH2 domain inhibitors from an unexplored mirror-image library of natural products.

Semisynthetic Src SH2 Domains Demonstrate Altered Phosphopeptide Specificity Induced by Incorporation of Unnatural Lysine Derivatives - Virdee, Satpal; Macmillan, Derek; Waksman, Gabriel (Chemistry & Biology, 2010)

Semisynthetic Src SH2 Domains Demonstrate Altered Phosphopeptide Specificity Induced by Incorporation of Unnatural Lysine Derivatives - Virdee, Satpal; Macmillan, Derek; Waksman, Gabriel (Chemistry & Biology, 2010)

Total synthesis of cytochrome b562 by native chemical ligation using a removable auxiliary - Low, D. W.; Hill, M. G.; Carrasco, M. R.; Kent, S. B. H.; Botti, P. (Proceedings of the National Academy of Sciences, 2001)

Total synthesis of cytochrome b562 by native chemical ligation using a removable auxiliary - Low, D. W.; Hill, M. G.; Carrasco, M. R.; Kent, S. B. H.; Botti, P. (Proceedings of the National Academy of Sciences, 2001)

Chemical Synthesis of HMGA1a Proteins with Post-translational Modifications via Ser/Thr Ligation - Li, Tianlu; Liu, Heng; Li, Xuechen (Organic Letters, 2016)

Chemical Synthesis of HMGA1a Proteins with Post-translational Modifications via Ser/Thr Ligation - Li, Tianlu; Liu, Heng; Li, Xuechen (Organic Letters, 2016)

Use of Serine/Threonine Ligation for the Total Chemical Synthesis of HMGA1a Protein with Site‐Specific Lysine Acetylations - Liu, Heng; Liu, Han; Li, Xuechen (ChemPlusChem, 2019)

Use of Serine/Threonine Ligation for the Total Chemical Synthesis of HMGA1a Protein with Site‐Specific Lysine Acetylations - Liu, Heng; Liu, Han; Li, Xuechen (ChemPlusChem, 2019)

A comparative study of synthetic and semisynthetic approaches for ligating the epidermal growth factor to a bivalent scaffold: Native Chemical Ligation of Synthetic and Semisynthetic EGF - Gell, Anna Lena; Groysbeck, Nadja; Becker, Christian F. W.; Conibear, Anne C. (Journal of Peptide Science, 2017)

A comparative study of synthetic and semisynthetic approaches for ligating the epidermal growth factor to a bivalent scaffold: Native Chemical Ligation of Synthetic and Semisynthetic EGF - Gell, Anna Lena; Groysbeck, Nadja; Becker, Christian F. W.; Conibear, Anne C. (Journal of Peptide Science, 2017)

Ixonnexin from Tick Saliva Promotes Fibrinolysis by Interacting with Plasminogen and Tissue-Type Plasminogen Activator, and Prevents Arterial Thrombosis - Assumpção, Teresa C.; Mizurini, Daniella M.; Ma, Dongying; Monteiro, Robson Q.; Ahlstedt, Sydney; Reyes, Morayma; Kotsyfakis, Michail; Mather, Thomas N.; Andersen, John F.; Lukszo, Jan; Ribeiro, José M. C.; Francischetti, Ivo M. B. (Scientific Reports, 2018)

Ixonnexin from Tick Saliva Promotes Fibrinolysis by Interacting with Plasminogen and Tissue-Type Plasminogen Activator, and Prevents Arterial Thrombosis - Assumpção, Teresa C.; Mizurini, Daniella M.; Ma, Dongying; Monteiro, Robson Q.; Ahlstedt, Sydney; Reyes, Morayma; Kotsyfakis, Michail; Mather, Thomas N.; Andersen, John F.; Lukszo, Jan; Ribeiro, José M. C.; Francischetti, Ivo M. B. (Scientific Reports, 2018)

Synthesis and structural insight into ESX-1 Substrate Protein C, an immunodominant Mycobacterium tuberculosis- secreted antigen: Synthesis and Structural Insight Into EspC - Son, Soo Jung; Harris, Paul W. R.; Squire, Chris J.; Baker, Edward N.; Brimble, Margaret A. (Biopolymers, 2016)

Synthesis and structural insight into ESX-1 Substrate Protein C, an immunodominant Mycobacterium tuberculosis- secreted antigen: Synthesis and Structural Insight Into EspC - Son, Soo Jung; Harris, Paul W. R.; Squire, Chris J.; Baker, Edward N.; Brimble, Margaret A. (Biopolymers, 2016)

Total Chemical Synthesis of Biologically Active Vascular Endothelial Growth Factor - Mandal, Kalyaneswar; Kent, Stephen B. H. (Angewandte Chemie International Edition, 2011)

Total Chemical Synthesis of Biologically Active Vascular Endothelial Growth Factor - Mandal, Kalyaneswar; Kent, Stephen B. H. (Angewandte Chemie International Edition, 2011)

Chemical synthesis and X-ray structure of a heterochiral {D-protein antagonist plus vascular endothelial growth factor} protein complex by racemic crystallography - Mandal, K.; Uppalapati, M.; Ault-Riche, D.; Kenney, J.; Lowitz, J.; Sidhu, S. S.; Kent, S. B. H. (Proceedings of the National Academy of Sciences, 2012)

Chemical synthesis and X-ray structure of a heterochiral {D-protein antagonist plus vascular endothelial growth factor} protein complex by racemic crystallography - Mandal, K.; Uppalapati, M.; Ault-Riche, D.; Kenney, J.; Lowitz, J.; Sidhu, S. S.; Kent, S. B. H. (Proceedings of the National Academy of Sciences, 2012)

One-pot native chemical ligation by combination of two orthogonal thioester precursors - Asahina, Yuya; Kawakami, Toru; Hojo, Hironobu (Chemical Communications, 2017)
We developed a one-pot peptide ligation method using two orthogonal thioester precursors and a protecting group for the ligation reaction between Asp and Cys. , We developed a one-pot peptide ligation method using two orthogonal thioester precursors and a protecting group for the ligation reaction between Asp and Cys. Combination of the two precursors facilitated the one-pot operation and yielded the entire polypeptide. The usefulness of this method was successfully demonstrated by the total synthesis of histone H4.

One-pot native chemical ligation by combination of two orthogonal thioester precursors - Asahina, Yuya; Kawakami, Toru; Hojo, Hironobu (Chemical Communications, 2017)
We developed a one-pot peptide ligation method using two orthogonal thioester precursors and a protecting group for the ligation reaction between Asp and Cys. , We developed a one-pot peptide ligation method using two orthogonal thioester precursors and a protecting group for the ligation reaction between Asp and Cys. Combination of the two precursors facilitated the one-pot operation and yielded the entire polypeptide. The usefulness of this method was successfully demonstrated by the total synthesis of histone H4.

Histone H4-K16 Acetylation Controls Chromatin Structure and Protein Interactions - Shogren-Knaak, M. (Science, 2006)

Histone H4-K16 Acetylation Controls Chromatin Structure and Protein Interactions - Shogren-Knaak, M. (Science, 2006)

Palladium-Assisted Removal of a Solubilizing Tag from a Cys Side Chain To Facilitate Peptide and Protein Synthesis - Maity, Suman Kumar; Mann, Guy; Jbara, Muhammad; Laps, Shay; Kamnesky, Guy; Brik, Ashraf (Organic Letters, 2016)

Palladium-Assisted Removal of a Solubilizing Tag from a Cys Side Chain To Facilitate Peptide and Protein Synthesis - Maity, Suman Kumar; Mann, Guy; Jbara, Muhammad; Laps, Shay; Kamnesky, Guy; Brik, Ashraf (Organic Letters, 2016)

Synthesis of Grb2 SH2 Domain Proteins for Mirror-Image Screening Systems - Noguchi, Taro; Ishiba, Hiroyuki; Honda, Kaori; Kondoh, Yasumitsu; Osada, Hiroyuki; Ohno, Hiroaki; Fujii, Nobutaka; Oishi, Shinya (Bioconjugate Chemistry, 2017)

Synthesis of Grb2 SH2 Domain Proteins for Mirror-Image Screening Systems - Noguchi, Taro; Ishiba, Hiroyuki; Honda, Kaori; Kondoh, Yasumitsu; Osada, Hiroyuki; Ohno, Hiroaki; Fujii, Nobutaka; Oishi, Shinya (Bioconjugate Chemistry, 2017)

Total Chemical Synthesis of Human Psoriasin by Native Chemical Ligation † - Li, Xiangqun; de Leeuw, Erik; Lu, Wuyuan (Biochemistry, 2005)

Total Chemical Synthesis of Human Psoriasin by Native Chemical Ligation † - Li, Xiangqun; de Leeuw, Erik; Lu, Wuyuan (Biochemistry, 2005)

Both the cis-trans equilibrium and isomerization dynamics of a single proline amide modulate 2-microglobulin amyloid assembly - Torbeev, V. Y.; Hilvert, D. (Proceedings of the National Academy of Sciences, 2013)

Both the cis-trans equilibrium and isomerization dynamics of a single proline amide modulate 2-microglobulin amyloid assembly - Torbeev, V. Y.; Hilvert, D. (Proceedings of the National Academy of Sciences, 2013)

Modular Total Chemical Synthesis of a Human Immunodeficiency Virus Type 1 Protease - Johnson, Erik C. B.; Malito, Enrico; Shen, Yuequan; Rich, Dan; Tang, Wei-Jen; Kent, Stephen B. H. (Journal of the American Chemical Society, 2007)

Chemical synthesis of the HPV16 E7 protein - Filippov, Dmitri V.; Welters, Marij J.P.; Valentijn, A. Rob P.M.; Melief, Cornelis J.M.; van der Marel, Gijsbert A.; van der Burg, Sjoerd H.; Overkleeft, Herman S.; Drijfhout, Jan W. (Tetrahedron Letters, 2006)

Modular Total Chemical Synthesis of a Human Immunodeficiency Virus Type 1 Protease - Johnson, Erik C. B.; Malito, Enrico; Shen, Yuequan; Rich, Dan; Tang, Wei-Jen; Kent, Stephen B. H. (Journal of the American Chemical Society, 2007)

Protein chemical synthesis by serine and threonine ligation - Zhang, Y.; Xu, C.; Lam, H. Y.; Lee, C. L.; Li, X. (Proceedings of the National Academy of Sciences, 2013)

Chemical synthesis of the HPV16 E7 protein - Filippov, Dmitri V.; Welters, Marij J.P.; Valentijn, A. Rob P.M.; Melief, Cornelis J.M.; van der Marel, Gijsbert A.; van der Burg, Sjoerd H.; Overkleeft, Herman S.; Drijfhout, Jan W. (Tetrahedron Letters, 2006)

Protein chemical synthesis by serine and threonine ligation - Zhang, Y.; Xu, C.; Lam, H. Y.; Lee, C. L.; Li, X. (Proceedings of the National Academy of Sciences, 2013)

Total Chemical Synthesis of the Integral Membrane Protein Influenza A Virus M2: Role of Its C-Terminal Domain in Tetramer Assembly † - Kochendoerfer, Gerd G.; Salom, David; Lear, James D.; Wilk-Orescan, Rosemarie; Kent, Stephen B. H.; DeGrado, William F. (Biochemistry, 1999)

Total synthesis of biotinylated N domain of human hepatocyte growth factor - Raibaut, Laurent; Vicogne, Jérome; Leclercq, Bérénice; Drobecq, Hervé; Desmet, Rémi; Melnyk, Oleg (Bioorganic & Medicinal Chemistry, 2013)

Total Chemical Synthesis of the Integral Membrane Protein Influenza A Virus M2: Role of Its C-Terminal Domain in Tetramer Assembly † - Kochendoerfer, Gerd G.; Salom, David; Lear, James D.; Wilk-Orescan, Rosemarie; Kent, Stephen B. H.; DeGrado, William F. (Biochemistry, 1999)

A novel PEG-based solid support enables the synthesis of >50 amino-acid peptide thioesters and the total synthesis of a functional SUMO-1 peptide conjugate - Boll, Emmanuelle; Drobecq, Hervé; Ollivier, Nathalie; Raibaut, Laurent; Desmet, Rémi; Vicogne, Jérome; Melnyk, Oleg (Chem. Sci., 2014)
Bis(2-sulfanylethyl)amino PEG resin gives access to large peptide thioester segments and to functional SUMO-1 conjugates. , A bis(2-sulfanylethyl)amino PEG-based resin enabled the synthesis of large (∼50 Aa) SEA or thioester peptides using Fmoc-SPPS. These peptide segments permitted the first total synthesis of a 97 amino-acid long SUMO-1-SEA peptide thioester surrogate and of a functional and reversible SUMO-1 peptide conjugate.

Total synthesis of biotinylated N domain of human hepatocyte growth factor - Raibaut, Laurent; Vicogne, Jérome; Leclercq, Bérénice; Drobecq, Hervé; Desmet, Rémi; Melnyk, Oleg (Bioorganic & Medicinal Chemistry, 2013)

A novel PEG-based solid support enables the synthesis of >50 amino-acid peptide thioesters and the total synthesis of a functional SUMO-1 peptide conjugate - Boll, Emmanuelle; Drobecq, Hervé; Ollivier, Nathalie; Raibaut, Laurent; Desmet, Rémi; Vicogne, Jérome; Melnyk, Oleg (Chem. Sci., 2014)
Bis(2-sulfanylethyl)amino PEG resin gives access to large peptide thioester segments and to functional SUMO-1 conjugates. , A bis(2-sulfanylethyl)amino PEG-based resin enabled the synthesis of large (∼50 Aa) SEA or thioester peptides using Fmoc-SPPS. These peptide segments permitted the first total synthesis of a 97 amino-acid long SUMO-1-SEA peptide thioester surrogate and of a functional and reversible SUMO-1 peptide conjugate.

A Helping Hand to Overcome Solubility Challenges in Chemical Protein Synthesis - Jacobsen, Michael T.; Petersen, Mark E.; Ye, Xiang; Galibert, Mathieu; Lorimer, George H.; Aucagne, Vincent; Kay, Michael S. (Journal of the American Chemical Society, 2016)

A Helping Hand to Overcome Solubility Challenges in Chemical Protein Synthesis - Jacobsen, Michael T.; Petersen, Mark E.; Ye, Xiang; Galibert, Mathieu; Lorimer, George H.; Aucagne, Vincent; Kay, Michael S. (Journal of the American Chemical Society, 2016)

Chemical synthesis of membrane proteins by the removable backbone modification method - Tang, Shan; Zuo, Chao; Huang, Dong-Liang; Cai, Xiao-Ying; Zhang, Long-Hua; Tian, Chang-Lin; Zheng, Ji-Shen; Liu, Lei (Nature Protocols, 2017)

Chemical synthesis of membrane proteins by the removable backbone modification method - Tang, Shan; Zuo, Chao; Huang, Dong-Liang; Cai, Xiao-Ying; Zhang, Long-Hua; Tian, Chang-Lin; Zheng, Ji-Shen; Liu, Lei (Nature Protocols, 2017)

Channel activity of mirror-image M2 proton channel of influenza A virus is blocked by achiral or chiral inhibitors - Guo, Qing-Yan; Zhang, Long-Hua; Zuo, Chao; Huang, Dong-Liang; Wang, Zhipeng A.; Zheng, Ji-Shen; Tian, Chang-Lin (Protein & Cell, 2019)

Channel activity of mirror-image M2 proton channel of influenza A virus is blocked by achiral or chiral inhibitors - Guo, Qing-Yan; Zhang, Long-Hua; Zuo, Chao; Huang, Dong-Liang; Wang, Zhipeng A.; Zheng, Ji-Shen; Tian, Chang-Lin (Protein & Cell, 2019)

Cysteine/Penicillamine Ligation Independent of Terminal Steric Demands for Chemical Protein Synthesis - Tan, Yi; Li, Jiasheng; Jin, Kang; Liu, Jiamei; Chen, Ziyong; Yang, Jun; Li, Xuechen (Angewandte Chemie International Edition, 2020)

Cysteine/Penicillamine Ligation Independent of Terminal Steric Demands for Chemical Protein Synthesis - Tan, Yi; Li, Jiasheng; Jin, Kang; Liu, Jiamei; Chen, Ziyong; Yang, Jun; Li, Xuechen (Angewandte Chemie International Edition, 2020)

Chemical Synthesis of Native S‐Palmitoylated Membrane Proteins through Removable‐Backbone‐Modification‐Assisted Ser/Thr Ligation - Huang, Dong‐Liang; Montigny, Cédric; Zheng, Yong; Beswick, Veronica; Li, Ying; Cao, Xiu‐Xiu; Barbot, Thomas; Jaxel, Christine; Liang, Jun; Xue, Min; Tian, Chang‐Lin; Jamin, Nadège; et al. (Angewandte Chemie International Edition, 2020)

Chemical Synthesis of Native S‐Palmitoylated Membrane Proteins through Removable‐Backbone‐Modification‐Assisted Ser/Thr Ligation - Huang, Dong‐Liang; Montigny, Cédric; Zheng, Yong; Beswick, Veronica; Li, Ying; Cao, Xiu‐Xiu; Barbot, Thomas; Jaxel, Christine; Liang, Jun; Xue, Min; Tian, Chang‐Lin; Jamin, Nadège; et al. (Angewandte Chemie International Edition, 2020)

Semisynthesis of an evasin from tick saliva reveals a critical role of tyrosine sulfation for chemokine binding and inhibition - Franck, Charlotte; Foster, Simon R.; Johansen-Leete, Jason; Chowdhury, Sayeeda; Cielesh, Michelle; Bhusal, Ram Prasad; Mackay, Joel P.; Larance, Mark; Stone, Martin J.; Payne, Richard J. (Proceedings of the National Academy of Sciences, 2020)
Blood-feeding arthropods produce antiinflammatory salivary proteins called evasins that function through inhibition of chemokine-receptor signaling in the host. Herein, we show that the evasin ACA-01 from the Amblyomma cajennense tick can be posttranslationally sulfated at two tyrosine residues, albeit as a mixture of sulfated variants. Homogenously sulfated variants of the proteins were efficiently assembled via a semisynthetic native chemical ligation strategy. Sulfation significantly improved the binding affinity of ACA-01 for a range of proinflammatory chemokines and enhanced the ability of ACA-01 to inhibit chemokine signaling through cognate receptors. Comparisons of evasin sequences and structural data suggest that tyrosine sulfation serves as a receptor mimetic strategy for recognizing and suppressing the proinflammatory activity of a wide variety of mammalian chemokines. As such, the incorporation of this posttranslational modification (PTM) or mimics thereof into evasins may provide a strategy to optimize tick salivary proteins for antiinflammatory applications.

Semisynthesis of an evasin from tick saliva reveals a critical role of tyrosine sulfation for chemokine binding and inhibition - Franck, Charlotte; Foster, Simon R.; Johansen-Leete, Jason; Chowdhury, Sayeeda; Cielesh, Michelle; Bhusal, Ram Prasad; Mackay, Joel P.; Larance, Mark; Stone, Martin J.; Payne, Richard J. (Proceedings of the National Academy of Sciences, 2020)
Blood-feeding arthropods produce antiinflammatory salivary proteins called evasins that function through inhibition of chemokine-receptor signaling in the host. Herein, we show that the evasin ACA-01 from the Amblyomma cajennense tick can be posttranslationally sulfated at two tyrosine residues, albeit as a mixture of sulfated variants. Homogenously sulfated variants of the proteins were efficiently assembled via a semisynthetic native chemical ligation strategy. Sulfation significantly improved the binding affinity of ACA-01 for a range of proinflammatory chemokines and enhanced the ability of ACA-01 to inhibit chemokine signaling through cognate receptors. Comparisons of evasin sequences and structural data suggest that tyrosine sulfation serves as a receptor mimetic strategy for recognizing and suppressing the proinflammatory activity of a wide variety of mammalian chemokines. As such, the incorporation of this posttranslational modification (PTM) or mimics thereof into evasins may provide a strategy to optimize tick salivary proteins for antiinflammatory applications.

A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligation - Hou, Wen; Liu, Lei; Zhang, Xiaohong; Liu, Chuanfa (Royal Society Open Science, 2018)
Sequential peptide ligation strategy becomes more and more important in large protein or long peptides chemical synthesis due to the limited peptide/protein size obtained by solid phase synthesis of individual peptides or even one-step peptide ligation. Herein, we developed an alternative method which could perform the sequential peptide ligation of several segments from N to C direction based on the combined use of thioacid capture ligation and native chemical ligation. The sweet protein monellin was produced through this strategy on a scale of multi-milligrams.

A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligation - Hou, Wen; Liu, Lei; Zhang, Xiaohong; Liu, Chuanfa (Royal Society Open Science, 2018)
Sequential peptide ligation strategy becomes more and more important in large protein or long peptides chemical synthesis due to the limited peptide/protein size obtained by solid phase synthesis of individual peptides or even one-step peptide ligation. Herein, we developed an alternative method which could perform the sequential peptide ligation of several segments from N to C direction based on the combined use of thioacid capture ligation and native chemical ligation. The sweet protein monellin was produced through this strategy on a scale of multi-milligrams.

Traceless Preparation of C-Terminal α-Ketoacids for Chemical Protein Synthesis by α-Ketoacid-Hydroxylamine Ligation: Synthesis of SUMO2/3 - Wucherpfennig, Thomas G.; Pattabiraman, Vijaya R.; Limberg, Felix R. P.; Ruiz-Rodríguez, Javier; Bode, Jeffrey W. (Angewandte Chemie International Edition, 2014)

Traceless Preparation of C-Terminal α-Ketoacids for Chemical Protein Synthesis by α-Ketoacid-Hydroxylamine Ligation: Synthesis of SUMO2/3 - Wucherpfennig, Thomas G.; Pattabiraman, Vijaya R.; Limberg, Felix R. P.; Ruiz-Rodríguez, Javier; Bode, Jeffrey W. (Angewandte Chemie International Edition, 2014)

Synthesis of a Double Transmembrane Domain Fragment of Ste2p by Native Chemical Ligation - Balambika, Rema; Inui, Tatsuya; Sargsyan, Hasmik; Arshava, Boris; Cohen, Leah S.; Ding, Fa Xiang; Becker, Jeffrey M.; Naider, Fred (International Journal of Peptide Research and Therapeutics, 2007)

Synthesis of a Double Transmembrane Domain Fragment of Ste2p by Native Chemical Ligation - Balambika, Rema; Inui, Tatsuya; Sargsyan, Hasmik; Arshava, Boris; Cohen, Leah S.; Ding, Fa Xiang; Becker, Jeffrey M.; Naider, Fred (International Journal of Peptide Research and Therapeutics, 2007)

Construction of a Ca 2+ -Gated Artificial Channel by Fusing Alamethicin with a Calmodulin-Derived Extramembrane Segment - Noshiro, Daisuke; Sonomura, Kazuhiro; Yu, Hao-Hsin; Imanishi, Miki; Asami, Koji; Futaki, Shiroh (Bioconjugate Chemistry, 2013)

Construction of a Ca 2+ -Gated Artificial Channel by Fusing Alamethicin with a Calmodulin-Derived Extramembrane Segment - Noshiro, Daisuke; Sonomura, Kazuhiro; Yu, Hao-Hsin; Imanishi, Miki; Asami, Koji; Futaki, Shiroh (Bioconjugate Chemistry, 2013)

Rapid Additive-Free Selenocystine–Selenoester Peptide Ligation - Mitchell, Nicholas J.; Malins, Lara R.; Liu, Xuyu; Thompson, Robert E.; Chan, Bun; Radom, Leo; Payne, Richard J. (Journal of the American Chemical Society, 2015)

Rapid Additive-Free Selenocystine–Selenoester Peptide Ligation - Mitchell, Nicholas J.; Malins, Lara R.; Liu, Xuyu; Thompson, Robert E.; Chan, Bun; Radom, Leo; Payne, Richard J. (Journal of the American Chemical Society, 2015)

Development of a dual-functional conjugate of antigenic peptide and Fc-III mimetics (DCAF) for targeted antibody blocking - Zhang, Lin; Shen, Hao; Gong, Yiyi; Pang, Xiaojing; Yi, Meiqi; Guo, Lin; Li, Jin; Arroyo, Sam; Lu, Xin; Ovchinnikov, Sergey; Cheng, Gong; Liu, Xudong; et al. (Chemical Science, 2019)
Long peptide DCAF enables high selectivity to target harmful antibodies, providing new thoughts for antibody-induced disease intervention. , Targeted antibody blocking enables characterization of binding sites on immunoglobulin G (IgG), and can efficiently eliminate harmful antibodies from organisms. In this report, we present a novel peptide—denoted as a dual-functional conjugate of antigenic peptide and Fc-III mimetics (DCAF)—for targeted blocking of antibodies. Synthesis of DCAF was achieved by native chemical ligation, and the molecule consists of three functional parts: a specific antigenic peptide, a linker and the Fc-III mimetic peptide, which has a high affinity toward the Fc region of IgG molecules. We demonstrate that DCAF binds the cognate antibody with high selectivity by simultaneously binding to the Fab and Fc regions of IgG. Animal experiments revealed that DCAF molecules diminish the antibody-dependent enhancement effect in a dengue virus infection model, and rescue the acetylcholine receptor by inhibiting the complement cascade in a myasthenia gravis model. These results suggest that DCAFs could have utility in the development of new therapeutics against harmful antibodies.

Development of a dual-functional conjugate of antigenic peptide and Fc-III mimetics (DCAF) for targeted antibody blocking - Zhang, Lin; Shen, Hao; Gong, Yiyi; Pang, Xiaojing; Yi, Meiqi; Guo, Lin; Li, Jin; Arroyo, Sam; Lu, Xin; Ovchinnikov, Sergey; Cheng, Gong; Liu, Xudong; et al. (Chemical Science, 2019)
Long peptide DCAF enables high selectivity to target harmful antibodies, providing new thoughts for antibody-induced disease intervention. , Targeted antibody blocking enables characterization of binding sites on immunoglobulin G (IgG), and can efficiently eliminate harmful antibodies from organisms. In this report, we present a novel peptide—denoted as a dual-functional conjugate of antigenic peptide and Fc-III mimetics (DCAF)—for targeted blocking of antibodies. Synthesis of DCAF was achieved by native chemical ligation, and the molecule consists of three functional parts: a specific antigenic peptide, a linker and the Fc-III mimetic peptide, which has a high affinity toward the Fc region of IgG molecules. We demonstrate that DCAF binds the cognate antibody with high selectivity by simultaneously binding to the Fab and Fc regions of IgG. Animal experiments revealed that DCAF molecules diminish the antibody-dependent enhancement effect in a dengue virus infection model, and rescue the acetylcholine receptor by inhibiting the complement cascade in a myasthenia gravis model. These results suggest that DCAFs could have utility in the development of new therapeutics against harmful antibodies.

Aspartic Acid Forming α-Ketoacid–Hydroxylamine (KAHA) Ligations with ( S )-4,4-Difluoro-5-oxaproline - Baldauf, Simon; Ogunkoya, Ayodele O.; Boross, Gábor N.; Bode, Jeffrey W. (The Journal of Organic Chemistry, 2020)

Aspartic Acid Forming α-Ketoacid–Hydroxylamine (KAHA) Ligations with ( S )-4,4-Difluoro-5-oxaproline - Baldauf, Simon; Ogunkoya, Ayodele O.; Boross, Gábor N.; Bode, Jeffrey W. (The Journal of Organic Chemistry, 2020)

N-Methylcysteine-mediated total chemical synthesis of ubiquitin thioester - Erlich, Lesly A.; Kumar, K. S. Ajish; Haj-Yahya, Mahmood; Dawson, Philip E.; Brik, Ashraf (Organic & Biomolecular Chemistry, 2010)

N-Methylcysteine-mediated total chemical synthesis of ubiquitin thioester - Erlich, Lesly A.; Kumar, K. S. Ajish; Haj-Yahya, Mahmood; Dawson, Philip E.; Brik, Ashraf (Organic & Biomolecular Chemistry, 2010)

A straightforward method for automated Fmoc-based synthesis of bio-inspired peptide crypto-thioesters - Terrier, Victor P.; Adihou, Hélène; Arnould, Mathieu; Delmas, Agnès F.; Aucagne, Vincent (Chemical Science, 2016)
A bio-inspired method for the synthesis of peptide thioester surrogates for native chemical ligation was developed. The process can be fully automated and does not require postsynthetic steps. , Despite recent advances, the direct Fmoc-based solid phase synthesis of peptide α-thioesters for the convergent synthesis of proteins via native chemical ligation (NCL) remains a challenge in the field. We herein report a simple and general methodology, enabling access to peptide thioester surrogates. A novel C-terminal N -(2-hydroxybenzyl)cysteine thioesterification device based on an amide-to-thioester rearrangement was developed, and the resulting peptide crypto-thioesters can be directly used in NCL reactions with fast N → S shift kinetics at neutral pH. These fast kinetics arise from our bio-inspired design, via intein-like intramolecular catalysis. Due to a well-positioned phenol moiety, an impressive >50 fold increase in the kinetic rate is observed compared to an O -methylated derivative. Importantly, the synthesis of this new device can be fully automated using inexpensive commercially available materials and does not require any post-synthetic steps prior to NCL. We successfully applied this new method to the synthesis of two long naturally-occurring cysteine-rich peptide sequences.

A straightforward method for automated Fmoc-based synthesis of bio-inspired peptide crypto-thioesters - Terrier, Victor P.; Adihou, Hélène; Arnould, Mathieu; Delmas, Agnès F.; Aucagne, Vincent (Chemical Science, 2016)
A bio-inspired method for the synthesis of peptide thioester surrogates for native chemical ligation was developed. The process can be fully automated and does not require postsynthetic steps. , Despite recent advances, the direct Fmoc-based solid phase synthesis of peptide α-thioesters for the convergent synthesis of proteins via native chemical ligation (NCL) remains a challenge in the field. We herein report a simple and general methodology, enabling access to peptide thioester surrogates. A novel C-terminal N -(2-hydroxybenzyl)cysteine thioesterification device based on an amide-to-thioester rearrangement was developed, and the resulting peptide crypto-thioesters can be directly used in NCL reactions with fast N → S shift kinetics at neutral pH. These fast kinetics arise from our bio-inspired design, via intein-like intramolecular catalysis. Due to a well-positioned phenol moiety, an impressive >50 fold increase in the kinetic rate is observed compared to an O -methylated derivative. Importantly, the synthesis of this new device can be fully automated using inexpensive commercially available materials and does not require any post-synthetic steps prior to NCL. We successfully applied this new method to the synthesis of two long naturally-occurring cysteine-rich peptide sequences.

Synthesis and Utility of β-Selenophenylalanine and β-Selenoleucine in Diselenide–Selenoester Ligation - Wang, Xiaoyi; Corcilius, Leo; Premdjee, Bhavesh; Payne, Richard J. (The Journal of Organic Chemistry, 2020)

Synthesis and Utility of β-Selenophenylalanine and β-Selenoleucine in Diselenide–Selenoester Ligation - Wang, Xiaoyi; Corcilius, Leo; Premdjee, Bhavesh; Payne, Richard J. (The Journal of Organic Chemistry, 2020)

Semisynthesis of NaK, a Na + and K + Conducting Ion Channel - Linn, Kellie M.; Derebe, Mehabaw G.; Jiang, Youxing; Valiyaveetil, Francis I. (Biochemistry, 2010)

Semisynthesis of NaK, a Na + and K + Conducting Ion Channel - Linn, Kellie M.; Derebe, Mehabaw G.; Jiang, Youxing; Valiyaveetil, Francis I. (Biochemistry, 2010)

Total Synthesis of the α-Subunit of Human Glycoprotein Hormones: Toward Fully Synthetic Homogeneous Human Follicle-Stimulating Hormone - Aussedat, Baptiste; Fasching, Bernhard; Johnston, Eric; Sane, Neeraj; Nagorny, Pavel; Danishefsky, Samuel J. (Journal of the American Chemical Society, 2012)

Total Synthesis of the α-Subunit of Human Glycoprotein Hormones: Toward Fully Synthetic Homogeneous Human Follicle-Stimulating Hormone - Aussedat, Baptiste; Fasching, Bernhard; Johnston, Eric; Sane, Neeraj; Nagorny, Pavel; Danishefsky, Samuel J. (Journal of the American Chemical Society, 2012)

Development of a strategy for the total chemical synthesis of an allergenic protein: the peach LTP Pru p 3: CHEMICAL SYNTHESIS OF THE ALLERGENIC PEACH LIPID TRANSFER PROTEIN - Buhler, Sofie; Akkerdaas, Jaap H.; A. Pertinhez, Thelma; Van Ree, Ronald; Dossena, Arnaldo; Sforza, Stefano; Tedeschi, Tullia (Journal of Peptide Science, 2017)

Development of a strategy for the total chemical synthesis of an allergenic protein: the peach LTP Pru p 3: CHEMICAL SYNTHESIS OF THE ALLERGENIC PEACH LIPID TRANSFER PROTEIN - Buhler, Sofie; Akkerdaas, Jaap H.; A. Pertinhez, Thelma; Van Ree, Ronald; Dossena, Arnaldo; Sforza, Stefano; Tedeschi, Tullia (Journal of Peptide Science, 2017)

Accelerated Protein Synthesis via One-Pot Ligation-Deselenization Chemistry - Mitchell, Nicholas J.; Sayers, Jessica; Kulkarni, Sameer S.; Clayton, Daniel; Goldys, Anna M.; Ripoll-Rozada, Jorge; Barbosa Pereira, Pedro José; Chan, Bun; Radom, Leo; Payne, Richard J. (Chem, 2017)

Accelerated Protein Synthesis via One-Pot Ligation-Deselenization Chemistry - Mitchell, Nicholas J.; Sayers, Jessica; Kulkarni, Sameer S.; Clayton, Daniel; Goldys, Anna M.; Ripoll-Rozada, Jorge; Barbosa Pereira, Pedro José; Chan, Bun; Radom, Leo; Payne, Richard J. (Chem, 2017)

Phosphorylation of the WWOX protein regulates its interaction with p73 - Lahav, Noa; Rotem-Bamberger, Shahar; Fahoum, Jamal; Dodson, Emma-Joy; Kraus, Yael; Mousa, Reem; Metanis, Norman; Friedler, Assaf; Schueler-Furman, Ora (ChemBioChem, 2020)

Synthesis of full length PB1-F2 influenza A virus proteins from ‘Spanish flu’ and ‘bird flu’ - Röder, René; Bruns, Karsten; Sharma, Alok; Eissmann, André; Hahn, Friedrich; Studtrucker, Nicole; Fossen, Torgils; Wray, Victor; Henklein, Peter; Schubert, Ulrich (Journal of Peptide Science, 2008)

Phosphorylation of the WWOX protein regulates its interaction with p73 - Lahav, Noa; Rotem-Bamberger, Shahar; Fahoum, Jamal; Dodson, Emma-Joy; Kraus, Yael; Mousa, Reem; Metanis, Norman; Friedler, Assaf; Schueler-Furman, Ora (ChemBioChem, 2020)

One-Pot Semisynthesis of Exon 1 of the Huntingtin Protein: New Tools for Elucidating the Role of Posttranslational Modifications in the Pathogenesis of Huntington’s Disease - Ansaloni, Annalisa; Wang, Zhe-Ming; Jeong, Jae Sun; Ruggeri, Francesco Simone; Dietler, Giovanni; Lashuel, Hilal A. (Angewandte Chemie International Edition, 2014)

Synthesis of full length PB1-F2 influenza A virus proteins from ‘Spanish flu’ and ‘bird flu’ - Röder, René; Bruns, Karsten; Sharma, Alok; Eissmann, André; Hahn, Friedrich; Studtrucker, Nicole; Fossen, Torgils; Wray, Victor; Henklein, Peter; Schubert, Ulrich (Journal of Peptide Science, 2008)

N-terminal Huntingtin (Htt) phosphorylation is a molecular switch regulating Htt aggregation, helical conformation, internalization, and nuclear targeting - DeGuire, Sean M.; Ruggeri, Francesco S.; Fares, Mohamed-Bilal; Chiki, Anass; Cendrowska, Urszula; Dietler, Giovanni; Lashuel, Hilal A. (Journal of Biological Chemistry, 2018)

One-Pot Semisynthesis of Exon 1 of the Huntingtin Protein: New Tools for Elucidating the Role of Posttranslational Modifications in the Pathogenesis of Huntington’s Disease - Ansaloni, Annalisa; Wang, Zhe-Ming; Jeong, Jae Sun; Ruggeri, Francesco Simone; Dietler, Giovanni; Lashuel, Hilal A. (Angewandte Chemie International Edition, 2014)

N-terminal Huntingtin (Htt) phosphorylation is a molecular switch regulating Htt aggregation, helical conformation, internalization, and nuclear targeting - DeGuire, Sean M.; Ruggeri, Francesco S.; Fares, Mohamed-Bilal; Chiki, Anass; Cendrowska, Urszula; Dietler, Giovanni; Lashuel, Hilal A. (Journal of Biological Chemistry, 2018)

Insulin-induced gene 1 (INSIG1) inhibits HIV-1 production by degrading Gag via activity of the ubiquitin ligase TRC8 - Zhang, You; Lu, Jing; Ma, Jing; Liu, Xinqi (Journal of Biological Chemistry, 2019)

Insulin-induced gene 1 (INSIG1) inhibits HIV-1 production by degrading Gag via activity of the ubiquitin ligase TRC8 - Zhang, You; Lu, Jing; Ma, Jing; Liu, Xinqi (Journal of Biological Chemistry, 2019)

Semi-Synthesis and Analysis of Chemically Modified Zif268 Zinc-Finger Domains - Fehr, Friederike; Nadler, André; Brodhun, Florian; Feussner, Ivo; Diederichsen, Ulf (ChemistryOpen, 2012)

Multiple Synthetic Routes to the Mini-Protein Omomyc and Coiled-Coil Domain Truncations - Brown, Zachary Z.; Mapelli, Claudio; Farasat, Iman; Shoultz, Alycia V.; Johnson, Scott A.; Orvieto, Federica; Santoprete, Alessia; Bianchi, Elisabetta; McCracken, Amy Bittner; Chen, Kuanchang; Zhu, Xiaohong; Demma, Mark J.; et al. (The Journal of Organic Chemistry, 2020)

Ligation of Expressed Protein α-Hydrazides via Genetic Incorporation of an α-Hydroxy Acid - Li, Yi-Ming; Yang, Mai-Yun; Huang, Yi-Chao; Li, Yi-Tong; Chen, Peng R.; Liu, Lei (ACS Chemical Biology, 2012)

Imidazole-Aided Native Chemical Ligation: Imidazole as a One-Pot Desulfurization-Amenable Non-Thiol-Type Alternative to 4-Mercaptophenylacetic Acid - Sakamoto, Ken; Tsuda, Shugo; Mochizuki, Masayoshi; Nohara, Yukie; Nishio, Hideki; Yoshiya, Taku (Chemistry - A European Journal, 2016)

Chemical synthesis of human adiponectin(19–107) bearing post-translational glycosylation - Mochizuki, Masayoshi; Taichi, Misako; Hibino, Hajime; Takuwa, Ayako; Yoshida, Takuya; Ohkubo, Tadayasu; Nishiuchi, Yuji (Tetrahedron Letters, 2014)

Traceless β-mercaptan-assisted activation of valinyl benzimidazolinones in peptide ligations - Wang, Yinglu; Han, Lin; Yuan, Ning; Wang, Hanxuan; Li, Hongxing; Liu, Jinrong; Chen, Huan; Zhang, Qiang; Dong, Suwei (Chemical Science, 2018)
An internal activation strategy-enabled traceless ligation at sterically hindered Val-Xaa site is accomplished under thiol additive-free conditions assisted by a β-mercaptan on the C-terminal valine residue. , Peptidyl thioesters or their surrogates with C-terminal β-branched hydrophobic amino acid residues usually exhibit poor reactivities in ligation reactions. Thus, activation using exogenous additives is required to ensure an acceptable reaction efficiency. Herein, we report a traceless ligation at Val-Xaa sites under mild thiol additive-free reaction conditions, whereby the introduction of β-mercaptan on the C-terminal valine residue effectively activates the otherwise unreactive N -acyl-benzimidazolinone (Nbz), and enables the use of a one-pot ligation–desulfurization strategy to generate the desired peptide products. The orthogonality between β-thiovaline-Nbz and a conventional alkyl thioester, as well as the convenient access to the former from readily available penicillamine, also allowed expedited assembly of the peptidic hormone β-LPH and hPTH analogues, based on a kinetically controlled one-pot three-segment ligation and desulfurization strategy.

Semi-Synthesis and Analysis of Chemically Modified Zif268 Zinc-Finger Domains - Fehr, Friederike; Nadler, André; Brodhun, Florian; Feussner, Ivo; Diederichsen, Ulf (ChemistryOpen, 2012)

Diselenide–selenoester ligation for chemical protein synthesis - Kulkarni, Sameer S.; Watson, Emma E.; Premdjee, Bhavesh; Conde-Frieboes, Kilian W.; Payne, Richard J. (Nature Protocols, 2019)

Multiple Synthetic Routes to the Mini-Protein Omomyc and Coiled-Coil Domain Truncations - Brown, Zachary Z.; Mapelli, Claudio; Farasat, Iman; Shoultz, Alycia V.; Johnson, Scott A.; Orvieto, Federica; Santoprete, Alessia; Bianchi, Elisabetta; McCracken, Amy Bittner; Chen, Kuanchang; Zhu, Xiaohong; Demma, Mark J.; et al. (The Journal of Organic Chemistry, 2020)

Toward Homogeneous Erythropoietin: Application of Metal-Free Dethiylation in the Chemical Synthesis of the Ala79-Arg166 Glycopeptide Domain - Dong, Suwei; Shang, Shiying; Tan, Zhongping; Danishefsky, Samuel J. (Israel Journal of Chemistry, 2011)

Ligation of Expressed Protein α-Hydrazides via Genetic Incorporation of an α-Hydroxy Acid - Li, Yi-Ming; Yang, Mai-Yun; Huang, Yi-Chao; Li, Yi-Tong; Chen, Peng R.; Liu, Lei (ACS Chemical Biology, 2012)

Imidazole-Aided Native Chemical Ligation: Imidazole as a One-Pot Desulfurization-Amenable Non-Thiol-Type Alternative to 4-Mercaptophenylacetic Acid - Sakamoto, Ken; Tsuda, Shugo; Mochizuki, Masayoshi; Nohara, Yukie; Nishio, Hideki; Yoshiya, Taku (Chemistry - A European Journal, 2016)

Chemical synthesis of human adiponectin(19–107) bearing post-translational glycosylation - Mochizuki, Masayoshi; Taichi, Misako; Hibino, Hajime; Takuwa, Ayako; Yoshida, Takuya; Ohkubo, Tadayasu; Nishiuchi, Yuji (Tetrahedron Letters, 2014)

Synthesis of neurosecretory protein GM composed of 88 amino acid residues by native chemical ligation - Masuda, Keiko; Furumitsu, Megumi; Ooyama, Haruka; Iwakoshi-Ukena, Eiko; Ukena, Kazuyoshi (Tetrahedron Letters, 2016)

Traceless β-mercaptan-assisted activation of valinyl benzimidazolinones in peptide ligations - Wang, Yinglu; Han, Lin; Yuan, Ning; Wang, Hanxuan; Li, Hongxing; Liu, Jinrong; Chen, Huan; Zhang, Qiang; Dong, Suwei (Chemical Science, 2018)
An internal activation strategy-enabled traceless ligation at sterically hindered Val-Xaa site is accomplished under thiol additive-free conditions assisted by a β-mercaptan on the C-terminal valine residue. , Peptidyl thioesters or their surrogates with C-terminal β-branched hydrophobic amino acid residues usually exhibit poor reactivities in ligation reactions. Thus, activation using exogenous additives is required to ensure an acceptable reaction efficiency. Herein, we report a traceless ligation at Val-Xaa sites under mild thiol additive-free reaction conditions, whereby the introduction of β-mercaptan on the C-terminal valine residue effectively activates the otherwise unreactive N -acyl-benzimidazolinone (Nbz), and enables the use of a one-pot ligation–desulfurization strategy to generate the desired peptide products. The orthogonality between β-thiovaline-Nbz and a conventional alkyl thioester, as well as the convenient access to the former from readily available penicillamine, also allowed expedited assembly of the peptidic hormone β-LPH and hPTH analogues, based on a kinetically controlled one-pot three-segment ligation and desulfurization strategy.

Total chemical synthesis by native chemical ligation of the all-D immunoglobulin-like domain 2 of Axl - De Rosa, Lucia; Di Stasi, Rossella; D'Andrea, Luca Domenico (Tetrahedron, 2019)

Palladium‐Mediated Cleavage of Proteins with Thiazolidine‐Modified Backbone in Live Cells - Mann, Guy; Satish, Gandhesiri; Meledin, Roman; Vamisetti, Ganga B.; Brik, Ashraf (Angewandte Chemie International Edition, 2019)

Influenza A virus protein PB1-F2: synthesis and characterization of the biologically active full length protein and related peptides - Henklein, Peter; Bruns, Karsten; Nimtz, Manfred; Wray, Victor; Tessmer, Uwe; Schubert, Ulrich (Journal of Peptide Science, 2005)

Diselenide–selenoester ligation for chemical protein synthesis - Kulkarni, Sameer S.; Watson, Emma E.; Premdjee, Bhavesh; Conde-Frieboes, Kilian W.; Payne, Richard J. (Nature Protocols, 2019)

Semisynthesis of a Glycosylated Im7 Analogue for Protein Folding Studies - Hackenberger, Christian P. R.; Friel, Claire T.; Radford, Sheena E.; Imperiali, Barbara (Journal of the American Chemical Society, 2005)

Toward Homogeneous Erythropoietin: Application of Metal-Free Dethiylation in the Chemical Synthesis of the Ala79-Arg166 Glycopeptide Domain - Dong, Suwei; Shang, Shiying; Tan, Zhongping; Danishefsky, Samuel J. (Israel Journal of Chemistry, 2011)

Total chemical synthesis of human proinsulin - Luisier, Samuel; Avital-Shmilovici, Michal; Weiss, Michael A.; Kent, Stephen B. H. (Chemical Communications, 2010)

Synthesis of neurosecretory protein GM composed of 88 amino acid residues by native chemical ligation - Masuda, Keiko; Furumitsu, Megumi; Ooyama, Haruka; Iwakoshi-Ukena, Eiko; Ukena, Kazuyoshi (Tetrahedron Letters, 2016)

Total chemical synthesis by native chemical ligation of the all-D immunoglobulin-like domain 2 of Axl - De Rosa, Lucia; Di Stasi, Rossella; D'Andrea, Luca Domenico (Tetrahedron, 2019)

HIV-1 Tat protein directly induces mitochondrial membrane permeabilization and inactivates cytochrome c oxidase - Lecoeur, H; Borgne-Sanchez, A; Chaloin, O; El-Khoury, R; Brabant, M; Langonné, A; Porceddu, M; Brière, J-J; Buron, N; Rebouillat, D; Péchoux, C; Deniaud, A; et al. (Cell Death & Disease, 2012)

Palladium‐Mediated Cleavage of Proteins with Thiazolidine‐Modified Backbone in Live Cells - Mann, Guy; Satish, Gandhesiri; Meledin, Roman; Vamisetti, Ganga B.; Brik, Ashraf (Angewandte Chemie International Edition, 2019)

Influenza A virus protein PB1-F2: synthesis and characterization of the biologically active full length protein and related peptides - Henklein, Peter; Bruns, Karsten; Nimtz, Manfred; Wray, Victor; Tessmer, Uwe; Schubert, Ulrich (Journal of Peptide Science, 2005)

Semisynthesis of a Glycosylated Im7 Analogue for Protein Folding Studies - Hackenberger, Christian P. R.; Friel, Claire T.; Radford, Sheena E.; Imperiali, Barbara (Journal of the American Chemical Society, 2005)

Protein Synthesis Assisted by Native Chemical Ligation at Leucine - Harpaz, Ziv; Siman, Peter; Kumar, K. S. Ajish; Brik, Ashraf (ChemBioChem, 2010)

Side-chain assisted ligation in protein synthesis - Ajish Kumar, K.S.; Harpaz, Ziv; Haj-Yahya, Mahmood; Brik, Ashraf (Bioorganic & Medicinal Chemistry Letters, 2009)

Total chemical synthesis of human proinsulin - Luisier, Samuel; Avital-Shmilovici, Michal; Weiss, Michael A.; Kent, Stephen B. H. (Chemical Communications, 2010)

Deciphering a Molecular Mechanism of Neonatal Diabetes Mellitus by the Chemical Synthesis of a Protein Diastereomer, [d-Ala B8 ]Human Proinsulin - Avital-Shmilovici, Michal; Whittaker, Jonathan; Weiss, Michael A.; Kent, Stephen B. H. (Journal of Biological Chemistry, 2014)

HIV-1 Tat protein directly induces mitochondrial membrane permeabilization and inactivates cytochrome c oxidase - Lecoeur, H; Borgne-Sanchez, A; Chaloin, O; El-Khoury, R; Brabant, M; Langonné, A; Porceddu, M; Brière, J-J; Buron, N; Rebouillat, D; Péchoux, C; Deniaud, A; et al. (Cell Death & Disease, 2012)

Protein Synthesis Assisted by Native Chemical Ligation at Leucine - Harpaz, Ziv; Siman, Peter; Kumar, K. S. Ajish; Brik, Ashraf (ChemBioChem, 2010)

Side-chain assisted ligation in protein synthesis - Ajish Kumar, K.S.; Harpaz, Ziv; Haj-Yahya, Mahmood; Brik, Ashraf (Bioorganic & Medicinal Chemistry Letters, 2009)

Deciphering a Molecular Mechanism of Neonatal Diabetes Mellitus by the Chemical Synthesis of a Protein Diastereomer, [d-Ala B8 ]Human Proinsulin - Avital-Shmilovici, Michal; Whittaker, Jonathan; Weiss, Michael A.; Kent, Stephen B. H. (Journal of Biological Chemistry, 2014)

Semi-synthesis of a HGF/SF kringle one (K1) domain scaffold generates a potent in vivo MET receptor agonist - Simonneau, Claire; Bérénice Leclercq, Bérénice Leclercq; Mougel, Alexandra; Adriaenssens, Eric; Paquet, Charlotte; Raibaut, Laurent; Ollivier, Nathalie; Drobecq, Hervé; Marcoux, Julien; Cianférani, Sarah; Tulasne, David; de Jonge, Hugo; et al. (Chemical Science, 2015)
Clustering of synthetic biotinylated K1 domain from HGF/SF by streptavidin is sufficient to generate a potent MET agonist. , The development of MET receptor agonists is an important goal in regenerative medicine, but is limited by the complexity and incomplete understanding of its interaction with HGF/SF (Hepatocyte Growth Factor/Scatter Factor). NK1 is a natural occurring agonist comprising the N-terminal (N) and the first kringle (K1) domains of HGF/SF. In the presence of heparin, NK1 can self-associate into a “head to tail” dimer which is considered as the minimal structural module able to trigger MET dimerization and activation whereas isolated K1 and N domains showed a weak or a complete lack of agonistic activity respectively. Starting from these structural and biological observations, we investigated whether it was possible to recapitulate the biological properties of NK1 using a new molecular architecture of isolated N or K1 domains. Therefore, we engineered multivalent N or K1 scaffolds by combining synthetic and homogeneous site-specifically biotinylated N and K1 domains (NB and K1B) and streptavidin (S). NB alone or in complex failed to activate MET signaling and to trigger cellular phenotypes. Importantly and to the contrary of K1B alone, the semi-synthetic K1B/S complex mimicked NK1 MET agonist activity in cell scattering, morphogenesis and survival phenotypic assays. Impressively, K1B/S complex stimulated in vivo angiogenesis and, when injected in mice, protected the liver against fulminant hepatitis in a MET dependent manner whereas NK1 and HGF were substantially less potent. These data reveal that without N domain, proper multimerization of K1 domain is a promising strategy for the rational design of powerful MET agonists.

Selenoglutaredoxin as a Glutathione Peroxidase Mimic - Casi, Giulio; Roelfes, Gerard; Hilvert, Donald (ChemBioChem, 2008)

Semi-synthesis of a HGF/SF kringle one (K1) domain scaffold generates a potent in vivo MET receptor agonist - Simonneau, Claire; Bérénice Leclercq, Bérénice Leclercq; Mougel, Alexandra; Adriaenssens, Eric; Paquet, Charlotte; Raibaut, Laurent; Ollivier, Nathalie; Drobecq, Hervé; Marcoux, Julien; Cianférani, Sarah; Tulasne, David; de Jonge, Hugo; et al. (Chemical Science, 2015)
Clustering of synthetic biotinylated K1 domain from HGF/SF by streptavidin is sufficient to generate a potent MET agonist. , The development of MET receptor agonists is an important goal in regenerative medicine, but is limited by the complexity and incomplete understanding of its interaction with HGF/SF (Hepatocyte Growth Factor/Scatter Factor). NK1 is a natural occurring agonist comprising the N-terminal (N) and the first kringle (K1) domains of HGF/SF. In the presence of heparin, NK1 can self-associate into a “head to tail” dimer which is considered as the minimal structural module able to trigger MET dimerization and activation whereas isolated K1 and N domains showed a weak or a complete lack of agonistic activity respectively. Starting from these structural and biological observations, we investigated whether it was possible to recapitulate the biological properties of NK1 using a new molecular architecture of isolated N or K1 domains. Therefore, we engineered multivalent N or K1 scaffolds by combining synthetic and homogeneous site-specifically biotinylated N and K1 domains (NB and K1B) and streptavidin (S). NB alone or in complex failed to activate MET signaling and to trigger cellular phenotypes. Importantly and to the contrary of K1B alone, the semi-synthetic K1B/S complex mimicked NK1 MET agonist activity in cell scattering, morphogenesis and survival phenotypic assays. Impressively, K1B/S complex stimulated in vivo angiogenesis and, when injected in mice, protected the liver against fulminant hepatitis in a MET dependent manner whereas NK1 and HGF were substantially less potent. These data reveal that without N domain, proper multimerization of K1 domain is a promising strategy for the rational design of powerful MET agonists.

A One-Pot Three-Segment Ligation Strategy for Protein Chemical Synthesis - Ollivier, Nathalie; Vicogne, Jérôme; Vallin, Aurélie; Drobecq, Hervé; Desmet, Rémi; El Mahdi, Ouafâa; Leclercq, Bérénice; Goormachtigh, Gautier; Fafeur, Véronique; Melnyk, Oleg (Angewandte Chemie International Edition, 2012)

Chemical Synthesis and Characterization of an Equinatoxin II(1–85) Analogue - Karas, John; Sani, Marc-Antoine; Separovic, Frances (Molecules, 2017)

Selenoglutaredoxin as a Glutathione Peroxidase Mimic - Casi, Giulio; Roelfes, Gerard; Hilvert, Donald (ChemBioChem, 2008)

A One-Pot Three-Segment Ligation Strategy for Protein Chemical Synthesis - Ollivier, Nathalie; Vicogne, Jérôme; Vallin, Aurélie; Drobecq, Hervé; Desmet, Rémi; El Mahdi, Ouafâa; Leclercq, Bérénice; Goormachtigh, Gautier; Fafeur, Véronique; Melnyk, Oleg (Angewandte Chemie International Edition, 2012)

Chemical Synthesis and Characterization of an Equinatoxin II(1–85) Analogue - Karas, John; Sani, Marc-Antoine; Separovic, Frances (Molecules, 2017)

Engineering of Therapeutic Polypeptides through Chemical Synthesis: Early Lessons from Human Parathyroid Hormone and Analogues - Dong, Suwei; Shang, Shiying; Li, Jianfeng; Tan, Zhongping; Dean, Thomas; Maeda, Akira; Gardella, Thomas J.; Danishefsky, Samuel J. (Journal of the American Chemical Society, 2012)

Engineering of Therapeutic Polypeptides through Chemical Synthesis: Early Lessons from Human Parathyroid Hormone and Analogues - Dong, Suwei; Shang, Shiying; Li, Jianfeng; Tan, Zhongping; Dean, Thomas; Maeda, Akira; Gardella, Thomas J.; Danishefsky, Samuel J. (Journal of the American Chemical Society, 2012)

Application of the logic of cysteine-free native chemical ligation to the synthesis of Human Parathyroid Hormone (hPTH) - Shang, S.; Tan, Z.; Danishefsky, S. J. (Proceedings of the National Academy of Sciences, 2011)

Application of the logic of cysteine-free native chemical ligation to the synthesis of Human Parathyroid Hormone (hPTH) - Shang, S.; Tan, Z.; Danishefsky, S. J. (Proceedings of the National Academy of Sciences, 2011)

Total chemical synthesis of human matrix Gla protein - Hackeng, T. M. (Protein Science, 2001)

Total chemical synthesis of human matrix Gla protein - Hackeng, T. M. (Protein Science, 2001)

Generation of the UFM1 Toolkit for Profiling UFM1-Specific Proteases and Ligases - Witting, Katharina F.; van der Heden van Noort, Gerbrand J.; Kofoed, Christian; Talavera Ormeño, Cami; el Atmioui, Dris; Mulder, Monique P. C.; Ovaa, Huib (Angewandte Chemie International Edition, 2018)

Generation of the UFM1 Toolkit for Profiling UFM1-Specific Proteases and Ligases - Witting, Katharina F.; van der Heden van Noort, Gerbrand J.; Kofoed, Christian; Talavera Ormeño, Cami; el Atmioui, Dris; Mulder, Monique P. C.; Ovaa, Huib (Angewandte Chemie International Edition, 2018)

Synthesis of theC-terminal region of opioid receptor like 1 in an SDS micelle by the native chemical ligation: effect of thiol additive and SDS concentration on ligation efficiency - Sato, Takeshi; Saito, Yasuhiro; Aimoto, Saburo (Journal of Peptide Science, 2005)

Synthesis of theC-terminal region of opioid receptor like 1 in an SDS micelle by the native chemical ligation: effect of thiol additive and SDS concentration on ligation efficiency - Sato, Takeshi; Saito, Yasuhiro; Aimoto, Saburo (Journal of Peptide Science, 2005)

Sequential α-Ketoacid-Hydroxylamine (KAHA) Ligations: Synthesis of C-Terminal Variants of the Modifier Protein UFM1 - Ogunkoya, Ayodele O.; Pattabiraman, Vijaya R.; Bode, Jeffrey W. (Angewandte Chemie International Edition, 2012)

Sequential α-Ketoacid-Hydroxylamine (KAHA) Ligations: Synthesis of C-Terminal Variants of the Modifier Protein UFM1 - Ogunkoya, Ayodele O.; Pattabiraman, Vijaya R.; Bode, Jeffrey W. (Angewandte Chemie International Edition, 2012)

Sugar-Assisted Ligation in Glycoprotein Synthesis - Yang, Yu-Ying; Ficht, Simon; Brik, Ashraf; Wong, Chi-Huey (Journal of the American Chemical Society, 2007)

Sugar-Assisted Ligation in Glycoprotein Synthesis - Yang, Yu-Ying; Ficht, Simon; Brik, Ashraf; Wong, Chi-Huey (Journal of the American Chemical Society, 2007)

Chemical Synthesis of A Pore-Forming Antimicrobial Protein, Caenopore-5, by Using Native Chemical Ligation at a Glu-Cys Site - Medini, Karima; Harris, Paul W. R.; Hards, Kiel; Dingley, Andrew J.; Cook, Gregory M.; Brimble, Margaret A. (ChemBioChem, 2015)

Chemical Synthesis of A Pore-Forming Antimicrobial Protein, Caenopore-5, by Using Native Chemical Ligation at a Glu-Cys Site - Medini, Karima; Harris, Paul W. R.; Hards, Kiel; Dingley, Andrew J.; Cook, Gregory M.; Brimble, Margaret A. (ChemBioChem, 2015)

Fmoc-Based Synthesis of Peptide- α Thioesters: Application to the Total Chemical Synthesis of a Glycoprotein by Native Chemical Ligation - Shin, Youngsook; Winans, Katharine A.; Backes, Bradley J.; Kent, Stephen B. H.; Ellman, Jonathan A.; Bertozzi, Carolyn R. (Journal of the American Chemical Society, 1999)

Fmoc-Based Synthesis of Peptide- α Thioesters: Application to the Total Chemical Synthesis of a Glycoprotein by Native Chemical Ligation - Shin, Youngsook; Winans, Katharine A.; Backes, Bradley J.; Kent, Stephen B. H.; Ellman, Jonathan A.; Bertozzi, Carolyn R. (Journal of the American Chemical Society, 1999)

Synthetic Seleno-Glutaredoxin 3 Analogues Are Highly Reducing Oxidoreductases with Enhanced Catalytic Efficiency - Metanis, Norman; Keinan, Ehud; Dawson, Philip E. (Journal of the American Chemical Society, 2006)

Synthetic Seleno-Glutaredoxin 3 Analogues Are Highly Reducing Oxidoreductases with Enhanced Catalytic Efficiency - Metanis, Norman; Keinan, Ehud; Dawson, Philip E. (Journal of the American Chemical Society, 2006)

Sulfation of the Human Cytomegalovirus Protein UL22A Enhances Binding to the Chemokine RANTES - Wang, Xiaoyi; Sanchez, Julie; Stone, Martin J.; Payne, Richard J. (Angewandte Chemie International Edition, 2017)

Sulfation of the Human Cytomegalovirus Protein UL22A Enhances Binding to the Chemokine RANTES - Wang, Xiaoyi; Sanchez, Julie; Stone, Martin J.; Payne, Richard J. (Angewandte Chemie International Edition, 2017)